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C4XLD5 · C4XLD5_SOLM1

Function

function

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide: step 1/2.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site251
Binding site321ATP (UniProtKB | ChEBI)
Binding site323Mg2+ 1 (UniProtKB | ChEBI)
Active site325Proton acceptor
Binding site346substrate
Binding site347Mg2+ 2 (UniProtKB | ChEBI)
Binding site481substrate
Binding site509Mg2+ 2 (UniProtKB | ChEBI)
Binding site743ATP (UniProtKB | ChEBI)
Binding site787ATP (UniProtKB | ChEBI)
Binding site790substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular Functionphosphoribosylformylglycinamidine synthase activity
Biological Process'de novo' IMP biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphoribosylformylglycinamidine synthase subunit PurL
  • EC number
  • Short names
    FGAM synthase
  • Alternative names
    • Formylglycinamide ribonucleotide amidotransferase subunit II
      (FGAR amidotransferase II
      ; FGAR-AT II
      )
    • Glutamine amidotransferase PurL
    • Phosphoribosylformylglycinamidine synthase subunit II

Gene names

    • Name
      purL
    • Ordered locus names
      DMR_35830

Organism names

Accessions

  • Primary accession
    C4XLD5

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain200-255Phosphoribosylformylglycinamidine synthase linker
Domain305-428PurM-like N-terminal
Domain445-596PurM-like C-terminal
Domain684-796PurM-like N-terminal
Domain830-972PurM-like C-terminal

Sequence similarities

Belongs to the FGAMS family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    997
  • Mass (Da)
    106,860
  • Last updated
    2009-07-28 v1
  • MD5 Checksum
    2F27394963914D969834C7F0C393E2C3
MLSRVAVGLRPSVRDVVGQRVAGKIQSELHLPVSAVSIVKIFTIDGLDPSAVEAVIAAGVLHDPVVQTASAAPITPAVPADWVIEVSFRPGVTDNEARTAKEAIALALGLDEAGRKGLAVYTGVQYHIAGLADQAAAEHVALDLLANDLLQRHDVKSAAQWTAEPGFPARTAKVTGKADSTVEVPDLFNMTEEQMLTLGRERTLALSLDEWWAIRAYFGLPGFKVRRVSWGLIHNPTDVELECLAQTWSEHCKHKIFSADITYVDNETGRTEVISSLYKSYIQRSTADIRAALGENDFCLSVFSDNAGVVKFTENLNLCIKVETHNSPSALDPYGGALTGIVGVNRDPMGTGIGANLLCNTDVFCFASPFHEGTLPPRLLHPRRVLEGVREGVEHGGNKSGVPTVNGSIVFDERFLGKPLVFCGTVGMLPATINGKPSHVKKALPGDYIVMVGGRIGKDGIHGATFSSEELHEGSPATAVQIGDPITQRRMYDCLMRARDMGLYNAITDNGAGGLSSSVGEMAQDAGGCELYLDRAPLKYDGLVPWEIFTSEAQERMTLAVPPDKFADFMRLAKEMGVEATELGNFTNSGYLRVYYGQRTVAYIDMDFLHGGTPRMALRAEWTRPVIARTEPKLPAEGHGALLKRMLGRLNICSKEYVVRQYDHEVKGGSAVKPMVGVRRDGPSDAGVLRPVLSRPEGVALSHGICPRYSDIDAYWMMAAAIDEAVRGAVAVGADPDRLAGVDNFCWCDPVESEKTPDGRYKLAQLVRANKALAHFCRAFMTPCVSGKDSMKNDYSGGGAKISIPPTVLFSVMGFVPDVSRVVTSDFKNAGDLVYVLGATHAELGASELAGELGFTSPDVPQVEAVSARRRYKTLHDAMMTGLVSACHDCSDGGLAVALAEMAIGGRLGADLDCDAAPGARGLTDLELLYSESASRLVVTVASENQAAFETLFAGQPCGLLGSVTQAPELTLRRGKAVLCAEPAEELARAFKATLDW

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP010904
EMBL· GenBank· DDBJ
BAH77074.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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