C3KUL4 · MURQ_CLOB6
- ProteinN-acetylmuramic acid 6-phosphate etherase
- GenemurQ
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids302 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D-lactate.
Miscellaneous
A lyase-type mechanism (elimination/hydration) is suggested for the cleavage of the lactyl ether bond of MurNAc 6-phosphate, with the formation of an alpha,beta-unsaturated aldehyde intermediate with (E)-stereochemistry, followed by the syn addition of water to give product.
Catalytic activity
- N-acetyl-D-muramate 6-phosphate + H2O = N-acetyl-D-glucosamine 6-phosphate + (R)-lactate
Pathway
Amino-sugar metabolism; N-acetylmuramate degradation.
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 86 | Proton donor | |||
Active site | 117 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | carbohydrate binding | |
Molecular Function | carbon-oxygen lyase activity | |
Molecular Function | enzyme binding | |
Molecular Function | enzyme inhibitor activity | |
Molecular Function | fructose-6-phosphate binding | |
Biological Process | amino sugar catabolic process | |
Biological Process | glucose homeostasis | |
Biological Process | N-acetylmuramic acid catabolic process | |
Biological Process | negative regulation of glucokinase activity | |
Biological Process | response to fructose |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameN-acetylmuramic acid 6-phosphate etherase
- EC number
- Short namesMurNAc-6-P etherase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Clostridiaceae > Clostridium
Accessions
- Primary accessionC3KUL4
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000202425 | 1-302 | N-acetylmuramic acid 6-phosphate etherase | ||
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length302
- Mass (Da)32,700
- Last updated2009-06-16 v1
- Checksum2473C719A7ED1DC3
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001083 EMBL· GenBank· DDBJ | ACQ55035.1 EMBL· GenBank· DDBJ | Genomic DNA |