C1E9Y5 · RTCB_MICCC

Function

function

Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.

Miscellaneous

Ligation probably proceeds through 3 nucleotidyl transfer steps, with 2',3'-cyclic phosphate termini being hydrolyzed to 3'-P termini in a step that precedes 3'-P activation with GMP. In the first nucleotidyl transfer step, RTCB reacts with GTP to form a covalent RTCB-histidine-GMP intermediate with release of PPi; in the second step, the GMP moiety is transferred to the RNA 3'-P; in the third step, the 5'-OH from the opposite RNA strand attacks the activated 3'-P to form a 3',5'-phosphodiester bond and release GMP.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 manganese ions per subunit.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site127Mn2+ 1 (UniProtKB | ChEBI)
Binding site130Mn2+ 1 (UniProtKB | ChEBI)
Binding site130Mn2+ 2 (UniProtKB | ChEBI)
Binding site234-238GMP (UniProtKB | ChEBI)
Binding site235Mn2+ 1 (UniProtKB | ChEBI)
Binding site267Mn2+ 2 (UniProtKB | ChEBI)
Binding site361Mn2+ 2 (UniProtKB | ChEBI)
Binding site361-362GMP (UniProtKB | ChEBI)
Binding site410-413GMP (UniProtKB | ChEBI)
Binding site417GMP (UniProtKB | ChEBI)
Active site436GMP-histidine intermediate
Binding site436-439GMP (UniProtKB | ChEBI)
Binding site512GMP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentnucleus
Cellular ComponenttRNA-splicing ligase complex
Molecular FunctionGTP binding
Molecular Functionmetal ion binding
Molecular FunctionRNA ligase (ATP) activity
Molecular FunctionRNA ligase (GTP) activity
Biological ProcesstRNA splicing, via endonucleolytic cleavage and ligation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RNA-splicing ligase RtcB homolog
  • EC number
  • Alternative names
    • 3'-phosphate/5'-hydroxy nucleic acid ligase

Gene names

    • ORF names
      MICPUN_59883

Organism names

Accessions

  • Primary accession
    C1E9Y5

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004072351-513RNA-splicing ligase RtcB homolog

Interaction

Subunit

Catalytic component of the tRNA-splicing ligase complex.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the RtcB family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    513
  • Mass (Da)
    56,239
  • Last updated
    2009-05-26 v1
  • Checksum
    186D1A358763F6F0
MGVMDLPGGRRTYAQEMEFLERVTPTQWRVREGFVPNMRVPGVFYVNKHLETLMFDELRQHVDRGDVGGFLPAVKQLANVACLPGIVSKSIALPDVHSGYGFAIGNVAAFDMSDPNAVVSPGGVGFDINCGVRVVRTNLHERDVTDIKEKLAQSLFDHIPVGVGSQGIIPTSPAGLEAALEMGMDWSLREGYAWAEDKEHCEEYGRMLNADPNKVSARAKKRGLPQMGTLGAGNHYAEIQVVDEIFDKHAADKMGIERLGQVMVMIHSGSRGLGHQVATDALTEMERAMARDGILVNDRQLACAKISSPEGQNYLSAMSCAANYAWVNRSSMTFLCRQAFAKMFDQTPDDLDMHVVYDVSHNIAKIEEHVVDGELKTLLVHRKGSTRAFPPHHPLIPVDYQYTGQPVLIGGTMGTCSYILTGTEKGMEETFGSTCHGAGRARSRNNSRNKLDYTEVLEKLKTKGIAIRVASPKLVMEEAPESYKDVTEVVNTCHDAGISKKAVKLRPIAVVKG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001328
EMBL· GenBank· DDBJ
ACO64763.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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