C1B2P8 · C1B2P8_RHOOB

Function

function

Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.

Catalytic activity

Cofactor

biotin (UniProtKB | Rhea| CHEBI:57586 )

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site117ATP (UniProtKB | ChEBI)
Binding site200ATP (UniProtKB | ChEBI)
Binding site235ATP (UniProtKB | ChEBI)
Active site292
Binding site533Mn2+ (UniProtKB | ChEBI)
Binding site605substrate
Binding site703Mn2+ (UniProtKB | ChEBI); via carbamate group
Binding site732Mn2+ (UniProtKB | ChEBI)
Binding site734Mn2+ (UniProtKB | ChEBI)
Binding site867substrate

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functionbiotin carboxylase activity
Molecular Functionmetal ion binding
Molecular Functionpyruvate carboxylase activity
Biological Processgluconeogenesis
Biological Processpyruvate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyruvate carboxylase
  • EC number

Gene names

    • Name
      pyc
    • Ordered locus names
      ROP_65680

Organism names

Accessions

  • Primary accession
    C1B2P8

Proteomes

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue703N6-carboxylysine
Modified residue1093N6-biotinyllysine

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-449Biotin carboxylation
Domain121-317ATP-grasp
Domain524-793Pyruvate carboxyltransferase
Domain1053-1127Lipoyl-binding

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,134
  • Mass (Da)
    121,643
  • Last updated
    2009-05-26 v1
  • Checksum
    C1FF033D96460A17
MFSKVLVANRGEIAIRAFRAAYELGAGTVAVFPYEDRNSIHRLKADESYQIGEEGHPVRAYLSVDEIVSAAKQAGADAIYPGYGFLSENPDLAAACAEAGIAFVGPSAEVLELTGNKARAIAAAKAAGLPVLASSEPSADVDELLAAAETMQFPLFVKAVAGGGGRGMRRVAERAQLKESIEAAAREAESAFGDPTVFLEQAVVDPRHIEVQILADGQGNVIHLFERDCSLQRRHQKVIELAPAPNLPEELRAKICADAVAFAKEINYACAGTVEFLLDTRGNHVFIEMNPRIQVEHTVTEEVTDVDLVQSQLRIASGETLADLGLSQDKITLRGAALQCRITTEDPANGFRPDTGRITAYRTPGGAGIRLDGGATLGAEVGAYFDSMLVKLTCRGRDFETAVARARRAVTEFRIRGVSTNIPFLQAVLDDPDFKAGRATTSFIEERPELLTLRSSADRGTKILSYLADVTVNKPHGERPSAVYPRDKLPQIDLSTPPPDGSRQKLLALGPEGFAKALREQKALAVTETTFRDAHQSLLATRVRTSGLLDVAGHVARLTPELLSIEAWGGATYDVALRFLHEDPWYRLSALREAVPNICLQMLLRGRNTVGYTPYPEKVTRAFVEEATNSGIDIFRIFDALNNVDQMRPAIDAVRETGTSVAEVALSYTGDLSNPHEKLYTLDYYLRLAEEIVEAGAHIIAIKDMAGLLRAPAATTLVTALRKNFDLPVHVHTHDTPGGQLATYLAAWQAGADAVDGASAAMAGTTSQPALSAIVAAAAHSEHDTGLDLQAVCDLEPYWEALRKVYAPFESGLPAPTGRVYTHEIPGGQLSNLRTQAVALGLGDKFEEVEAKYAAADRMLGRLVKVTPSSKVVGDLALHLVGSGASTEEFKDNPAKFDLPDSVVGFLRGELGTPPGGWPEPFRTRALEGRGEAKPEVPLSAEDEKALAGTSAERRATLNRLLFPGPTKEFLEHRDKYGDTSQLSANQFFYGLRRGDEHRVRLAQGVELLIGLEAISEPDERGYRTVMAILNGQLRPVSVRDRSISSEIPAAEKADKNNPGHVPAPFAGVVTLAVTEGQHVAAGDTIATIEAMKMEAAITAPRGGTVTRLAIGSVQQVEGGDLLAVVATGESASE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP011115
EMBL· GenBank· DDBJ
BAH54815.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp