C1A6H2 · DNLJ_GEMAT
- ProteinDNA ligase
- GeneligA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids689 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
Catalytic activity
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 40-44 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 89-90 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 122 | NAD+ (UniProtKB | ChEBI) | |||
Active site | 124 | N6-AMP-lysine intermediate | |||
Binding site | 145 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 182 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 300 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 325 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 419 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 422 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 437 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 442 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | DNA binding | |
Molecular Function | DNA ligase (NAD+) activity | |
Molecular Function | metal ion binding | |
Biological Process | base-excision repair, DNA ligation | |
Biological Process | DNA replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Gemmatimonadota > Gemmatimonadia > Gemmatimonadales > Gemmatimonadaceae > Gemmatimonas
Accessions
- Primary accessionC1A6H2
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000380387 | 1-689 | DNA ligase | ||
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 600-689 | BRCT | |||
Sequence similarities
Belongs to the NAD-dependent DNA ligase family. LigA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length689
- Mass (Da)74,174
- Last updated2009-05-26 v1
- Checksum55B6927E11591FA1
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP009153 EMBL· GenBank· DDBJ | BAH37832.1 EMBL· GenBank· DDBJ | Genomic DNA |