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C0ZNG9 · C0ZNG9_RHOE4

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

1978100200300400500600700800900
Type
IDPosition(s)Description
Site47Interaction with DNA
Site166Interaction with DNA
Site167Interaction with DNA
Site170Interaction with DNA
Site175Interaction with DNA
Site182Interaction with DNA
Active site337O-(5'-phospho-DNA)-tyrosine intermediate
Site339Interaction with DNA
Site540Interaction with DNA

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • Ordered locus names
      RER_05130

Organism names

Accessions

  • Primary accession
    C0ZNG9

Proteomes

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain17-141Toprim
Region190-195Interaction with DNA
Region749-789Disordered
Compositional bias767-781Basic residues
Region818-843Disordered
Region864-978Disordered
Compositional bias908-937Basic and acidic residues
Compositional bias938-969Basic residues

Sequence similarities

Belongs to the type IA topoisomerase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    978
  • Mass (Da)
    106,618
  • Last updated
    2009-05-26 v1
  • MD5 Checksum
    512B502BF8AE994F13242B88582221F5
MAKADNGSGQGASGNPRRLVIVESPTKARKIAPYLGKDYVVEASVGHIRDLPRGAADVPAKYKGESWARLGVNVDHDFEPLYVVSPEKKGKVAELKSLLKDADELFLATDPDREGEAIAWHLLETLKPKIPVRRMVFHEITEPAIRAAAEDTRELDTDLVDAQETRRILDRLYGYEVSPVLWKKVMPKLSAGRVQSVATRVIVQRERERMAFKSASYWDISATLDAGAEASPRSFGARLVSVDGSRVASGRDFGADGKLKGSAVTVLDEAYARRLAEALQGVDLTVSSAEDKPYTRKPYPPFMTSTLQQEAGRKLRFTSERAMRIAQRLYENGYITYMRTDSTTLSESAIAAARAQATELYGPEYVHPTPRQYTRKVKNAQEAHEAIRPAGDVFQTPGQLHSKLDSDEFKLYELIWQRTVASQMADARGTTLTLRISGTAGTGEDCTFSASGRTIVFAGFLKAYVESVDEEAGGQSDDAESRLPVLVKGQEVTATKLDPDGHSTNPPARYTEASLIKTLEELGIGRPSTYSSIIKTILDRGYVYKRGSALVPSWVAFAVIGLLEVHFGRLVDFDFTAGMEDDLDAIAGGREQRGNWLSSFYFGGETGAEGSVAREGGLKKMVGVNLEEIDAREVNSIRLFDDAEGREVHVRVGRFGPYLERMVQNPDDPEGDLISQRANLPDDLPPDELTPEYAEKLFSTPQEGRKLGVDPLTGHEIVAKEGRFGPYVTEILPEPPAEPEPDIVPVSAPEPEEISSGSTKTAVKKAAAKKAPAKKAAKKVAGPKPRTGSLLKSMDLATITLEDALRLLSLPRVVGVDPESKEEITAQNGRYGPYLKKGTDSRSLANEEQMFTVTLDEALKIYAEPKRRGRQASATPPLRELGVDPVSEKPMVIKDGRFGPYVTDGETNASLRKDDDVESISDARASELLADRRARGPVKKKAAAKKAPAKKAAAKKTAAKKAPAKKAAAKKAPAKKDS

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias767-781Basic residues
Compositional bias908-937Basic and acidic residues
Compositional bias938-969Basic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP008957
EMBL· GenBank· DDBJ
BAH31221.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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