C0RJF0 · QUEF_BRUMB
- ProteinNADPH-dependent 7-cyano-7-deazaguanine reductase
- GenequeF
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids155 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).
Catalytic activity
- 7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-deazaguanine + 3 H+ + 2 NADPH
Pathway
tRNA modification; tRNA-queuosine biosynthesis.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 53 | Thioimide intermediate | ||||
Sequence: C | ||||||
Active site | 60 | Proton donor | ||||
Sequence: D | ||||||
Binding site | 75-77 | substrate | ||||
Sequence: VES | ||||||
Binding site | 94-95 | substrate | ||||
Sequence: HE |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | preQ1 synthase activity | |
Biological Process | queuosine biosynthetic process | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADPH-dependent 7-cyano-7-deazaguanine reductase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Brucellaceae > Brucella/Ochrobactrum group > Brucella
Accessions
- Primary accessionC0RJF0
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000148668 | 1-155 | NADPH-dependent 7-cyano-7-deazaguanine reductase | |||
Sequence: MSENTIYSGLKQLGSHTDIPLTPEEAVLERVANPQEGTPYCVRFTAPEFSSLCPMTGQPDFAHLVIDYVPGKWLVESKSLKLFLFSFRNHGAFHEDCTVTIGKRLVDLLEPEWLRIGGYWYPRGGIPIDVFYQTGAAPLNVWIPEQGVANYRGRG |
Structure
Sequence
- Sequence statusComplete
- Length155
- Mass (Da)17,402
- Last updated2009-05-05 v1
- ChecksumBEC57D5171BBC051
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001488 EMBL· GenBank· DDBJ | ACO00958.1 EMBL· GenBank· DDBJ | Genomic DNA |