C0RH46 · PANB_BRUMB
- Protein3-methyl-2-oxobutanoate hydroxymethyltransferase
- GenepanB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids275 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.
Catalytic activity
- (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = (6S)-5,6,7,8-tetrahydrofolate + 2-dehydropantoate
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 49 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 49-50 | 3-methyl-2-oxobutanoate (UniProtKB | ChEBI) | ||||
Sequence: DS | ||||||
Binding site | 88 | 3-methyl-2-oxobutanoate (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 88 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 118 | 3-methyl-2-oxobutanoate (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 120 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 187 | Proton acceptor | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 3-methyl-2-oxobutanoate hydroxymethyltransferase activity | |
Molecular Function | metal ion binding | |
Biological Process | pantothenate biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3-methyl-2-oxobutanoate hydroxymethyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Brucellaceae > Brucella/Ochrobactrum group > Brucella
Accessions
- Primary accessionC0RH46
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000123371 | 1-275 | 3-methyl-2-oxobutanoate hydroxymethyltransferase | |||
Sequence: MSAPVTRKRLTPKVIQAMKGECPIVSLTAYTTPVARLLDPHCDLLLVGDSLGMVLYGMESTLAVTLDMMIMHGQAVMRGTSHACVIVDMPFGSYQESKEQAFRNAARVMQETGCDGVKLEGGEEMAETVAFLVRRGIPVFGHVGLMPQQVNTVGGFRSLGRGDDEAGKIRRDAQAIAQAGAFAVVIEGTVEPLAREITALIDIPTVGIGASSACDGQVLVSDDMLGLFQDFTPRFVKRFAHLAPQVSQAAEAYAEEVRARRFPGPEHVFGAKPGA |
Interaction
Subunit
Homodecamer; pentamer of dimers.
Structure
Sequence
- Sequence statusComplete
- Length275
- Mass (Da)29,418
- Last updated2009-05-05 v1
- ChecksumF73117B84258FE26
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001488 EMBL· GenBank· DDBJ | ACO00154.1 EMBL· GenBank· DDBJ | Genomic DNA |