C0RE67 · C0RE67_BRUMB
- ProteinD-alanine--D-alanine ligase
- Geneddl
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids308 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Cell wall formation.
Catalytic activity
- ATP + 2 D-alanine = ADP + D-alanyl-D-alanine + H+ + phosphate
Cofactor
Protein has several cofactor binding sites:
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 2 magnesium or manganese ions per subunit.
Pathway
Cell wall biogenesis; peptidoglycan biosynthesis.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 16 | |||||
Sequence: E | ||||||
Active site | 142 | |||||
Sequence: S | ||||||
Binding site | 252 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 269 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 269 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 271 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Active site | 280 | |||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | D-alanine-D-alanine ligase activity | |
Molecular Function | lyase activity | |
Molecular Function | metal ion binding | |
Biological Process | cell wall organization | |
Biological Process | peptidoglycan biosynthetic process | |
Biological Process | regulation of cell shape |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameD-alanine--D-alanine ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Brucellaceae > Brucella/Ochrobactrum group > Brucella
Accessions
- Primary accessionC0RE67
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 102-302 | ATP-grasp | ||||
Sequence: KKVAAAAGVVVAPSRLMNRFDIGSQHPMKPPYVVKPVREGSSFGVVIVKEDQPHPPQVIGSADWKYGDEVMVEGYIAGRELTCAVMGDRAMDVCEIMPVGYQFYDYDSKYVAGASTHVCPAKILPNIYQKIQTMALTAHRAIGCRGVSRSDFRFDDRFSEEGEVVWLEINTQPGMTPTSLVPDIAKAAGISFAELLSWMVE |
Sequence similarities
Belongs to the D-alanine--D-alanine ligase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length308
- Mass (Da)33,412
- Last updated2009-05-05 v1
- Checksum15EA7C52171453B8
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001488 EMBL· GenBank· DDBJ | ACO01189.1 EMBL· GenBank· DDBJ | Genomic DNA |