C0P9J6 · ADH1A_MAIZE
- ProteinAminoaldehyde dehydrogenase 1a
- GeneAMADH1A
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids505 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Dehydrogenase that catalyzes the oxidation of several aminoaldehydes (PubMed:23408433).
Metabolizes and detoxifies aldehyde products of polyamine degradation to non-toxic amino acids (Probable). Catalyzes the oxidation of 4-aminobutanal and 3-aminopropanal to 4-aminobutanoate and beta-alanine, respectively (PubMed:23408433).
Catalyzes the oxidation of 4-(trimethylamino)butanal and 4-guanidinobutanal to 4-trimethylammoniobutanoate and 4-guanidinobutanoate, respectively (PubMed:23408433).
Catalyzes the oxidation of betaine aldehyde to glycine betaine (PubMed:23408433).
Metabolizes and detoxifies aldehyde products of polyamine degradation to non-toxic amino acids (Probable). Catalyzes the oxidation of 4-aminobutanal and 3-aminopropanal to 4-aminobutanoate and beta-alanine, respectively (PubMed:23408433).
Catalyzes the oxidation of 4-(trimethylamino)butanal and 4-guanidinobutanal to 4-trimethylammoniobutanoate and 4-guanidinobutanoate, respectively (PubMed:23408433).
Catalyzes the oxidation of betaine aldehyde to glycine betaine (PubMed:23408433).
Dehydrogenase that catalyzes the oxidation of several aminoaldehydes (PubMed:21740525).
Catalyzes the oxidation of betaine aldehyde to glycine betaine (PubMed:21740525).
Catalyzes the oxidation of 4-(trimethylamino)butanal to 4-trimethylammoniobutanoate (PubMed:21740525).
Catalyzes the oxidation of betaine aldehyde to glycine betaine (PubMed:21740525).
Catalyzes the oxidation of 4-(trimethylamino)butanal to 4-trimethylammoniobutanoate (PubMed:21740525).
Catalytic activity
- 4-aminobutanal + H2O + NAD+ = 4-aminobutanoate + 2 H+ + NADHThis reaction proceeds in the forward direction.
- 3-aminopropanal + H2O + NAD+ = beta-alanine + 2 H+ + NADHThis reaction proceeds in the forward direction.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
28 μM | 4-aminobutanal | |||||
9 μM | 3-aminopropanal | |||||
6 μM | 4-(trimethylamino)butanal | |||||
3 μM | 4-guanidinobutanal | |||||
14 μM | betaine aldehyde | |||||
91 μM | NAD+ with 3-aminopropanal as substrate |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
11 nmol/sec/mg | with betaine aldehyde as substrate |
Pathway
Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine from betaine aldehyde: step 1/1.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 101 | Na+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 161-163 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: TPW | ||||||
Site | 164 | Transition state stabilizer | ||||
Sequence: N | ||||||
Binding site | 187-190 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: KPSE | ||||||
Binding site | 191 | Na+ (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 241-244 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: SFET | ||||||
Active site | 262 | Proton acceptor | ||||
Sequence: E | ||||||
Binding site | 262 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 296 | Nucleophile | ||||
Sequence: C | ||||||
Binding site | 395 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 461 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: W |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4-trimethylammoniobutyraldehyde dehydrogenase activity | |
Molecular Function | aminobutyraldehyde dehydrogenase activity | |
Molecular Function | betaine-aldehyde dehydrogenase activity | |
Molecular Function | gamma-guanidinobutyraldehyde dehydrogenase activity | |
Molecular Function | nucleotide binding | |
Molecular Function | protein homodimerization activity | |
Molecular Function | sodium ion binding | |
Biological Process | cellular detoxification of aldehyde | |
Biological Process | glycine betaine biosynthetic process from choline |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAminoaldehyde dehydrogenase 1a
- EC number
- Short namesZmAMADH1a
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > PACMAD clade > Panicoideae > Andropogonodae > Andropogoneae > Tripsacinae > Zea
Accessions
- Primary accessionC0P9J6
Proteomes
Genome annotation databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000454135 | 1-505 | Aminoaldehyde dehydrogenase 1a | |||
Sequence: MASPAMVPLRQLFVDGEWRPPAQGRRLPVVNPTTEAHIGEIPAGTAEDVDAAVAAARAALKRNRGRDWARAPGAVRAKYLRAIAAKVIERKPELAKLEALDCGKPYDEAAWDMDDVAGCFEYFADQAEALDKRQNSPVSLPMETFKCHLRREPIGVVGLITPWNYPLLMATWKIAPALAAGCTAVLKPSELASVTCLELADICKEVGLPSGVLNIVTGLGPDAGAPLSAHPDVDKVAFTGSFETGKKIMASAAPMVKPVTLELGGKSPIVVFDDVDIDKAVEWTLFGCFWTNGQICSATSRLLIHTKIAKKFNERMVAWAKNIKVSDPLEEGCRLGPVVSEGQYEKIKKFISNAKSQGATILTGGVRPAHLEKGFFIEPTIITDITTSMEIWREEVFGPVLCVKEFSTEDEAIELANDTQYGLAGAVISGDRERCQRLSEEIDAGCIWVNCSQPCFCQAPWGGNKRSGFGRELGEGGIDNYLSVKQVTEYISDEPWGWYQSPSKL |
Expression
Gene expression databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length505
- Mass (Da)54,913
- Last updated2009-05-05 v1
- Checksum5B3D693764642D8F
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A804NQX3 | A0A804NQX3_MAIZE | 100302679 | 393 | ||
A0A804NQX4 | A0A804NQX4_MAIZE | 100302679 | 413 | ||
A0A804NQX5 | A0A804NQX5_MAIZE | 100302679 | 476 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
GQ184593 EMBL· GenBank· DDBJ | ACS74867.1 EMBL· GenBank· DDBJ | mRNA | ||
CM000780 EMBL· GenBank· DDBJ | AQK52374.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT064965 EMBL· GenBank· DDBJ | ACN30841.1 EMBL· GenBank· DDBJ | mRNA |