C0NSF3 · THI4_AJECG
- ProteinThiamine thiazole synthase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids334 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.
Catalytic activity
- [ADP-thiazole synthase]-L-cysteine + glycine + NAD+ = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + 2 H+
Cofactor
Note: Binds 1 Fe cation per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 86 | substrate | |||
Binding site | 107-108 | substrate | |||
Binding site | 115 | substrate | |||
Binding site | 183 | substrate | |||
Binding site | 223 | substrate | |||
Binding site | 238 | substrate | |||
Binding site | 290 | substrate | |||
Binding site | 300-302 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | nucleus | |
Molecular Function | iron ion binding | |
Molecular Function | pentosyltransferase activity | |
Biological Process | thiamine biosynthetic process | |
Biological Process | thiazole biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameThiamine thiazole synthase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Onygenales > Ajellomycetaceae > Histoplasma
Accessions
- Primary accessionC0NSF3
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000415869 | 1-334 | Thiamine thiazole synthase | ||
Modified residue | 221 | 2,3-didehydroalanine (Cys) | |||
Post-translational modification
During the catalytic reaction, a sulfide is transferred from Cys-221 to a reaction intermediate, generating a dehydroalanine residue.
Interaction
Structure
Sequence
- Sequence statusComplete
- Length334
- Mass (Da)35,737
- Last updated2012-02-22 v2
- ChecksumCF3265D89DEB0CA0
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
GG663370 EMBL· GenBank· DDBJ | EEH05819.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation |