C0HLR8 · TX1_OCUSU
- ProteinKappa-lycotoxin-Os1a
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Insecticidal to house crickets (PubMed:33597864).
It induces an excitatory slow-onset impact that leads to irreversible spastic paralysis (PubMed:33597864).
It also modifies human voltage-gated potassium channel Kv1.5/KCNA5 (PubMed:33597864).
Most likely, it binds to the voltage-sensing domain of the channel, suggesting it does not block the pore but prevents its opening at physiological membrane potentials (PubMed:33597864).
The recombinant peptide binds to the channel in an irreversible manner and slows down the hKv1.5 current activation kinetics (PubMed:33597864).
It is not toxic to mice, when intracranially injected (at 0.5 ug/g mouse) (PubMed:33597864).
It induces an excitatory slow-onset impact that leads to irreversible spastic paralysis (PubMed:33597864).
It also modifies human voltage-gated potassium channel Kv1.5/KCNA5 (PubMed:33597864).
Most likely, it binds to the voltage-sensing domain of the channel, suggesting it does not block the pore but prevents its opening at physiological membrane potentials (PubMed:33597864).
The recombinant peptide binds to the channel in an irreversible manner and slows down the hKv1.5 current activation kinetics (PubMed:33597864).
It is not toxic to mice, when intracranially injected (at 0.5 ug/g mouse) (PubMed:33597864).
Miscellaneous
This toxin represents a concentration of ~60 ug per mg of O.supermirabilis dry crude venom.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | potassium channel regulator activity | |
Molecular Function | toxin activity |
Keywords
- Molecular function
Names & Taxonomy
Protein names
- Recommended nameKappa-lycotoxin-Os1a
- Short namesKappa-LCTX-Os1a
- Alternative names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Chelicerata > Arachnida > Araneae > Araneomorphae > Entelegynae > Lycosoidea > Lycosidae > Oculicosa
Accessions
- Primary accessionC0HLR8
Subcellular Location
Phenotypes & Variants
Toxic dose
PD50 is 0.05 ug/g (6.6 pmol/g) in house crickets (Acheta domesticus).
LD50 is <0.1 ug/g (<13.3 pmol/g) in house crickets (Acheta domesticus).
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000453031 | 1-64 | Kappa-lycotoxin-Os1a | ||
Disulfide bond | 10↔26 | ||||
Disulfide bond | 17↔56 | ||||
Disulfide bond | 19↔42 | ||||
Disulfide bond | 28↔40 | ||||
Keywords
- PTM
Expression
Tissue specificity
Expressed by the venom gland.
Structure
Family & Domains
Sequence similarities
Belongs to the neurotoxin 04 (omega-agtx) family. 01 (type I omega-agtx) subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length64
- Mass (Da)7,486
- Last updated2021-06-02 v1
- MD5 ChecksumAF39EF9BE6F325005B842683033CA00E
Features
Showing features for sequence uncertainty.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence uncertainty | 53 | Assigned by comparison with orthologs and mass difference | |||
Mass Spectrometry
Keywords
- Technical term