C0HLG3 · RNAG_CYCAE
- ProteinRibonuclease ageritin
- GeneAGT1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids156 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Fungal ribonuclease involved in fungal defense. Highly specific and highly toxic fungal endonuclease that cleaves a single phosphodiester bond in the 28S RNA of eukaryotic ribosomes at a universally conserved GAGA tetraloop of the sarcin-ricin loop (SRL). The damage of the SRL inhibits the binding of translation elongation factors and halts protein biosynthesis, ultimately resulting in the death of the target cells (PubMed:28232091, PubMed:30262416, PubMed:31444206).
Shows antitumor activity (PubMed:28232091, PubMed:30262416).
Exerts cytotoxicity and induces apoptosis towards rat glial cells and human glioma cells, and also displays some activity towards human neurolastoma cell lines (PubMed:28232091).
Shows a strong entomotoxicity against Aedes aegypti larvae, yet no nematotoxicity against nematodes (PubMed:31444206).
Shows antitumor activity (PubMed:28232091, PubMed:30262416).
Exerts cytotoxicity and induces apoptosis towards rat glial cells and human glioma cells, and also displays some activity towards human neurolastoma cell lines (PubMed:28232091).
Shows a strong entomotoxicity against Aedes aegypti larvae, yet no nematotoxicity against nematodes (PubMed:31444206).
Catalytic activity
Cofactor
Activity regulation
In contrast to most ribotoxins, activity is completely inhibited by EDTA.
Biotechnology
The strong larvicidal activity of ageritin makes this protein a promising candidate for novel biopesticide development.
Temperature Dependence
Highly thermostable. Has a melting temperature of 78 degrees Celsius at pH 7.4.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 98 | |||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | vacuolar lumen | |
Molecular Function | lyase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | RNA endonuclease activity | |
Molecular Function | rRNA endonuclease activity | |
Biological Process | killing of cells of another organism |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibonuclease ageritin
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Basidiomycota > Agaricomycotina > Agaricomycetes > Agaricomycetidae > Agaricales > Agaricineae > Bolbitiaceae > Cyclocybe
Accessions
- Primary accessionC0HLG3
- Secondary accessions
Subcellular Location
UniProt Annotation
GO Annotation
Note: Possibly sequestered into the vacuole to avoid its toxic activity on ribosomes.
Keywords
- Cellular component
Phenotypes & Variants
PTM/Processing
Features
Showing features for signal, chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-21 | |||||
Sequence: MSESSTFTTAVVPEGEGVAPM | ||||||
Chain | PRO_0000446086 | 22-156 | Ribonuclease ageritin | |||
Sequence: AETVQYYNSYSDASIASCAFVDSGKDKIDKTKLVTYTSRLAASPAYQKVVGVGLKTAAGSIVPYVRLDMDNTGKGIHFNATKLSDSSAKLAAVLKTTVSMTEAQRTQLYMEYIKGIENRSAQFIWDWWRTGKAPA | ||||||
Glycosylation | 100 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 139 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
PTM databases
Expression
Developmental stage
Highly Expressed during fruiting body formation.
Interaction
Subunit
Monomer.
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length156
- Mass (Da)16,910
- Last updated2021-02-10 v3
- ChecksumA18DDC3863F6BDE6
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 39 | in Ref. 4; AA sequence | ||||
Sequence: C → M | ||||||
Sequence conflict | 41 | in Ref. 4; AA sequence | ||||
Sequence: F → I | ||||||
Sequence conflict | 153 | in Ref. 5; AA sequence | ||||
Sequence: K → V | ||||||
Sequence conflict | 156 | in Ref. 5; AA sequence | ||||
Sequence: A → T |
Mass Spectrometry
Keywords
- Technical term