C0HLG3 · RNAG_CYCAE

Function

function

Fungal ribonuclease involved in fungal defense. Highly specific and highly toxic fungal endonuclease that cleaves a single phosphodiester bond in the 28S RNA of eukaryotic ribosomes at a universally conserved GAGA tetraloop of the sarcin-ricin loop (SRL). The damage of the SRL inhibits the binding of translation elongation factors and halts protein biosynthesis, ultimately resulting in the death of the target cells (PubMed:28232091, PubMed:30262416, PubMed:31444206).
Shows antitumor activity (PubMed:28232091, PubMed:30262416).
Exerts cytotoxicity and induces apoptosis towards rat glial cells and human glioma cells, and also displays some activity towards human neurolastoma cell lines (PubMed:28232091).
Shows a strong entomotoxicity against Aedes aegypti larvae, yet no nematotoxicity against nematodes (PubMed:31444206).

Catalytic activity

  • a 28S rRNA containing guanosine-adenosine pair + H2O = an [RNA fragment]-3'-adenosine-3'-phosphate + a 5'-a hydroxy-guanosine-3'-[RNA fragment].
    EC:4.6.1.23 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

In contrast to most ribotoxins, activity is completely inhibited by EDTA.

Biotechnology

The strong larvicidal activity of ageritin makes this protein a promising candidate for novel biopesticide development.

Temperature Dependence

Highly thermostable. Has a melting temperature of 78 degrees Celsius at pH 7.4.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site98

GO annotations

AspectTerm
Cellular Componentvacuolar lumen
Molecular Functionlyase activity
Molecular Functionmagnesium ion binding
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA endonuclease activity
Biological Processkilling of cells of another organism

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease ageritin
  • EC number
  • Alternative names
    • Ribotoxin

Gene names

    • Name
      AGT1
    • ORF names
      AAE3_01767

Organism names

Accessions

  • Primary accession
    C0HLG3
  • Secondary accessions
    • A0A5B9CUE1

Subcellular Location

Vacuole lumen
Note: Possibly sequestered into the vacuole to avoid its toxic activity on ribosomes.

Keywords

Phenotypes & Variants

PTM/Processing

Features

Showing features for signal, chain, glycosylation.

TypeIDPosition(s)Description
Signal1-21
ChainPRO_000044608622-156Ribonuclease ageritin
Glycosylation100N-linked (GlcNAc...) asparagine
Glycosylation139N-linked (GlcNAc...) asparagine

Keywords

PTM databases

Expression

Developmental stage

Highly Expressed during fruiting body formation.

Interaction

Subunit

Monomer.

Structure

Family & Domains

Sequence similarities

Belongs to the ribotoxin-like family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    156
  • Mass (Da)
    16,910
  • Last updated
    2021-02-10 v3
  • Checksum
    A18DDC3863F6BDE6
MSESSTFTTAVVPEGEGVAPMAETVQYYNSYSDASIASCAFVDSGKDKIDKTKLVTYTSRLAASPAYQKVVGVGLKTAAGSIVPYVRLDMDNTGKGIHFNATKLSDSSAKLAAVLKTTVSMTEAQRTQLYMEYIKGIENRSAQFIWDWWRTGKAPA

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict39in Ref. 4; AA sequence
Sequence conflict41in Ref. 4; AA sequence
Sequence conflict153in Ref. 5; AA sequence
Sequence conflict156in Ref. 5; AA sequence

Mass Spectrometry

Molecular mass is 14,802.84 Da. Determined by MALDI.
Molecular mass is 14,802.94 Da. Determined by MALDI.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MK411345
EMBL· GenBank· DDBJ
QEE04510.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp