C0HKG9 · TX1C_CHEPU

Function

function

Spider venom toxin that exhibits cytolytic activity by forming an alpha-helix across the membrane (PubMed:20657014).
Lethal to insect larvae (PubMed:20657014, PubMed:24717175).
Causes instant paralysis and death in the larvae of the flesh fly (S.carnaria) at doses of 20 ug/g, at doses of less than 10 ug/g causes reversible paralysis (PubMed:20657014).
Has cytolytic activity against insect Sf9 cells (PubMed:20657014).
Causes stable and irreversible depolarization of fly muscle fibers, leading to contracture at higher toxin concentrations (PubMed:20657014).
Destabilizes membranes (PubMed:20657014).

Caution

Characterization of function and toxicity was performed using CpTx1, a mixture of DELTA-miturgitoxin-Cp1a, DELTA-miturgitoxin-Cp1b and DELTA-miturgitoxin-Cp1c. While it is assumed that all three are active current data cannot prove this.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Cellular Componentmembrane
Cellular Componentother organism cell membrane
Molecular Functiontoxin activity
Biological Processkilling of cells of another organism

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    DELTA-miturgitoxin-Cp1c
  • Short names
    DELTA-MGTX-Cp1c
  • Alternative names
    • Toxin CpTx1
    • Toxin CpTx1c

Organism names

Accessions

  • Primary accession
    C0HKG9
  • Secondary accessions
    • D5GSJ8
    • D5GSJ9
    • D7FBA1
    • D7FBA3
    • P86381

Subcellular Location

Secreted
Target cell membrane
Note: Probably forms a transmembrane alpha-helix in the target cell membrane.

Keywords

Phenotypes & Variants

Toxic dose

LD50 is 10 ug/g on S.carnaria larvae.

PTM/Processing

Features

Showing features for signal, propeptide, chain, disulfide bond, modified residue.

TypeIDPosition(s)Description
Signal1-20
PropeptidePRO_000044015621-47
ChainPRO_000044015748-181DELTA-miturgitoxin-Cp1c
Disulfide bond51↔66
Disulfide bond58↔75
Disulfide bond65↔88
Disulfide bond77↔86
Disulfide bond115↔130
Disulfide bond122↔139
Disulfide bond129↔157
Disulfide bond141↔155
Modified residue181Tryptophan amide

Post-translational modification

Cleavage of the propeptide depends on the processing quadruplet motif (XXXR, with at least one of X being E).

Keywords

Expression

Tissue specificity

Expressed by the venom gland.

Structure

Family & Domains

Features

Showing features for motif, region.

TypeIDPosition(s)Description
Motif44-47Processing quadruplet motif
Region164-177Predicted alpha-helix

Domain

The presence of 'disulfide through disulfide knots' structurally defines this protein as a knottin. This toxin contains 2 'disulfide through disulfide knots' (By similarity).

Sequence similarities

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    183
  • Mass (Da)
    20,778
  • Last updated
    2017-06-07 v1
  • Checksum
    10C781D1695AD51E
MKFSLFFSVFFLAVLHACLSESEIDLEDEEHFMSSDSFLSEIQDESRGKTCIERNKECTNDRHGCCRGKIFKDKCTCVKSGKTEKCVCTQKKWAKIIESYIGDIPALPKPVDDKCVPKHADCSKRKDDCCKGGIFKYQCKCYDMYDDDGEKTDLCGCVSPVEHQAIEGALRIAKKLIGDRWGR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FN594517
EMBL· GenBank· DDBJ
CBH50811.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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