C0HKG9 · TX1C_CHEPU
- ProteinDELTA-miturgitoxin-Cp1c
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids183 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Spider venom toxin that exhibits cytolytic activity by forming an alpha-helix across the membrane (PubMed:20657014).
Lethal to insect larvae (PubMed:20657014, PubMed:24717175).
Causes instant paralysis and death in the larvae of the flesh fly (S.carnaria) at doses of 20 ug/g, at doses of less than 10 ug/g causes reversible paralysis (PubMed:20657014).
Has cytolytic activity against insect Sf9 cells (PubMed:20657014).
Causes stable and irreversible depolarization of fly muscle fibers, leading to contracture at higher toxin concentrations (PubMed:20657014).
Destabilizes membranes (PubMed:20657014).
Lethal to insect larvae (PubMed:20657014, PubMed:24717175).
Causes instant paralysis and death in the larvae of the flesh fly (S.carnaria) at doses of 20 ug/g, at doses of less than 10 ug/g causes reversible paralysis (PubMed:20657014).
Has cytolytic activity against insect Sf9 cells (PubMed:20657014).
Causes stable and irreversible depolarization of fly muscle fibers, leading to contracture at higher toxin concentrations (PubMed:20657014).
Destabilizes membranes (PubMed:20657014).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Cellular Component | membrane | |
Cellular Component | other organism cell membrane | |
Molecular Function | toxin activity | |
Biological Process | killing of cells of another organism |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameDELTA-miturgitoxin-Cp1c
- Short namesDELTA-MGTX-Cp1c
- Alternative names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Chelicerata > Arachnida > Araneae > Araneomorphae > Entelegynae > Entelegynae incertae sedis > Cheiracanthiidae > Cheiracanthium
Accessions
- Primary accessionC0HKG9
- Secondary accessions
Subcellular Location
UniProt Annotation
GO Annotation
Note: Probably forms a transmembrane alpha-helix in the target cell membrane.
Keywords
- Cellular component
Phenotypes & Variants
Toxic dose
LD50 is 10 ug/g on S.carnaria larvae.
PTM/Processing
Features
Showing features for signal, propeptide, chain, disulfide bond, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-20 | |||||
Sequence: MKFSLFFSVFFLAVLHACLS | ||||||
Propeptide | PRO_0000440156 | 21-47 | ||||
Sequence: ESEIDLEDEEHFMSSDSFLSEIQDESR | ||||||
Chain | PRO_0000440157 | 48-181 | DELTA-miturgitoxin-Cp1c | |||
Sequence: GKTCIERNKECTNDRHGCCRGKIFKDKCTCVKSGKTEKCVCTQKKWAKIIESYIGDIPALPKPVDDKCVPKHADCSKRKDDCCKGGIFKYQCKCYDMYDDDGEKTDLCGCVSPVEHQAIEGALRIAKKLIGDRW | ||||||
Disulfide bond | 51↔66 | |||||
Sequence: CIERNKECTNDRHGCC | ||||||
Disulfide bond | 58↔75 | |||||
Sequence: CTNDRHGCCRGKIFKDKC | ||||||
Disulfide bond | 65↔88 | |||||
Sequence: CCRGKIFKDKCTCVKSGKTEKCVC | ||||||
Disulfide bond | 77↔86 | |||||
Sequence: CVKSGKTEKC | ||||||
Disulfide bond | 115↔130 | |||||
Sequence: CVPKHADCSKRKDDCC | ||||||
Disulfide bond | 122↔139 | |||||
Sequence: CSKRKDDCCKGGIFKYQC | ||||||
Disulfide bond | 129↔157 | |||||
Sequence: CCKGGIFKYQCKCYDMYDDDGEKTDLCGC | ||||||
Disulfide bond | 141↔155 | |||||
Sequence: CYDMYDDDGEKTDLC | ||||||
Modified residue | 181 | Tryptophan amide | ||||
Sequence: W |
Post-translational modification
Cleavage of the propeptide depends on the processing quadruplet motif (XXXR, with at least one of X being E).
Keywords
- PTM
Expression
Tissue specificity
Expressed by the venom gland.
Structure
Family & Domains
Features
Showing features for motif, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 44-47 | Processing quadruplet motif | ||||
Sequence: DESR | ||||||
Region | 164-177 | Predicted alpha-helix | ||||
Sequence: QAIEGALRIAKKLI |
Domain
The presence of 'disulfide through disulfide knots' structurally defines this protein as a knottin. This toxin contains 2 'disulfide through disulfide knots' (By similarity).
Sequence similarities
Belongs to the neurotoxin 19 (CSTX) family. Double-CSTX subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length183
- Mass (Da)20,778
- Last updated2017-06-07 v1
- Checksum10C781D1695AD51E
Keywords
- Technical term