C0HK44 · LL3_LASLA

Function

function

Antimicrobial peptide which assumes an amphiphilic alpha-helix conformation upon contact with membranes (PubMed:19591185).
Insertion into membranes involves Trp-3 (By similarity).
Penetrates into cells once membrane has been permeated (PubMed:22100226).
Active against Gram-negative bacteria E.coli (MIC=1.4 uM), P.aeruginosa (MIC=18.7 uM) and Gram-positive bacteria S.aureus (MIC=3.9 uM) and B.subtilis (MIC=0.7 uM) (PubMed:19591185).
Has cytotoxic but no hemolytic activity (PubMed:19591185, PubMed:22100226).
Binds DNA in vitro (By similarity).
In the context of inflammation and cancer tests, a 3-residues C-terminally truncated lasioglossin-3 is weakly cytotoxic to normal cells, induces calcium signaling but does not impact cAMP production (PubMed:36548715).
In addition, this truncated peptide prevents LPS-induced nitric oxid (NO) synthesis but does not affect the IP3 signaling and pro-inflammatory activation of endothelial cells (PubMed:36548715).
This truncated peptide does not show significant antiproliferative activity on the breast cancer cell line MDA-MB-231 (PubMed:36548715).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Molecular FunctionDNA binding
Biological Processdefense response to bacterium

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Lasioglossin-3
  • Short names
    LL-III

Organism names

  • Taxonomic identifier
  • Organism
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Hymenoptera > Apocrita > Aculeata > Apoidea > Anthophila > Halictidae > Halictinae > Halictini > Lasioglossum > Evylaeus

Accessions

  • Primary accession
    C0HK44

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis1Reduced cytotoxicity.
Mutagenesis1-2Reduced activity against S.aureus and P.aeruginosa. No effect on cytotoxicity.
Mutagenesis6Reduced cytotoxicity.
Mutagenesis6-8No effect on cytotoxicity.
Mutagenesis8Slightly increased activity against S.aureus and B.subtilis, reduced activity against P.aeruginosa. Shows hemolytic activity. Slightly increased cytotoxicity.
Mutagenesis8Slightly increased activity against E.coli, reduced activity against S.aureus and P.aeruginosa. Shows hemolytic activity. Slightly decreased cytotoxicity.
Mutagenesis8Reduced antimicrobial activity except against E.coli. Almost complete loss of cytotoxicity.
Mutagenesis11Strongly reduced cytotoxicity.

PTM/Processing

Features

Showing features for peptide, modified residue.

TypeIDPosition(s)Description
PeptidePRO_00004376521-15Lasioglossin-3
Modified residue15Lysine amide

Post-translational modification

The C-terminal amidation is required for full activity.

Keywords

Expression

Tissue specificity

Expressed by the venom gland.

Family & Domains

Sequence similarities

Belongs to the lasioglossin-like family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    15
  • Mass (Da)
    1,766
  • Last updated
    2016-11-02 v1
  • Checksum
    2604A73CD4C7E7B6
VNWKKILGKIIKVVK

Mass Spectrometry

Molecular mass is 1,764.1 Da. Determined by Electrospray.

Keywords

Similar Proteins

Disclaimer

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