C0ERJ0 · C0ERJ0_NEIFL
- ProteintRNA modification GTPase MnmE
- GenemnmE
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids335 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.
Cofactor
Note: Binds 1 potassium ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 24 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 82 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 121 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 227 | K+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 227-232 | GTP (UniProtKB | ChEBI) | ||||
Sequence: NVGKSS | ||||||
Binding site | 231 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 246 | K+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 246-252 | GTP (UniProtKB | ChEBI) | ||||
Sequence: TDIAGTT | ||||||
Binding site | 248 | K+ (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 251 | K+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 252 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 271-274 | GTP (UniProtKB | ChEBI) | ||||
Sequence: DTAG | ||||||
Binding site | 329 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | metal ion binding | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA modification GTPase MnmE
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Neisseriales > Neisseriaceae > Neisseria
Accessions
- Primary accessionC0ERJ0
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 217-335 | TrmE-type G | ||||
Sequence: GMNVVLVGAPNVGKSSLLNALAGDDIAIVTDIAGTTRDTVREQITLDGVPVHIIDTAGLRETDDVVEQIGIERSRKAVSEADVALILIDPREGVNAKTQAILNSLPEGLKKSKSTTKPT |
Sequence similarities
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length335
- Mass (Da)35,334
- Last updated2009-05-05 v1
- Checksum308413A55F8CBD84