B9N2B0 · LISC2_POPTR

Function

function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N6-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site128[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site133[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site139[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site159[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site163[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site166[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site374[4Fe-4S] cluster 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchloroplast
Cellular Componentmitochondrion
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionlipoate synthase activity
Molecular Functionmetal ion binding
Biological Processlipoate biosynthetic process
Biological Processprotein lipoylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Lipoyl synthase 2, chloroplastic
  • EC number
  • Alternative names
    • Lipoate synthase 2
      (LS 2
      ; Lip-syn 2
      )
    • Lipoate synthase, plastidial 2
      (LIP1p 2
      )
    • Lipoic acid synthase 2

Gene names

    • Name
      LIP1P-2
    • ORF names
      POPTR_0019s13380g

Organism names

Accessions

  • Primary accession
    B9N2B0
  • Secondary accessions
    • U5FF64

Proteomes

Genome annotation databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for transit peptide, chain.

Type
IDPosition(s)Description
Transit peptide1-35Chloroplast
ChainPRO_000039886836-397Lipoyl synthase 2, chloroplastic

Expression

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for compositional bias, region, domain.

Type
IDPosition(s)Description
Compositional bias49-71Polar residues
Region49-85Disordered
Domain142-363Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    397
  • Mass (Da)
    43,638
  • Last updated
    2009-03-24 v1
  • Checksum
    FC77A8FC05480DA9
MIEQSLSKPSFSLSIPIPKAPKSKSSFFCSYSKIRCESVDYPSLTKIDAKHPQNSTTINNGSSSSASVDLKNNEKGPYPYPGGGKMGPYTGRDLNEKKPEWLRQRAPQGERFEEVKESISRLNLNTVCQEAQCPNIGECWNGGGDGIATATIMVLGDTCTRGCRFCAVKTSRTPPPPDPMEPLNTALAIASWGVDYIVITSVDRDDLSDGGSGHFAQTVRAMKELKPEIMVECLTSDFRGDLKAVDTLVHSGLDVFAHNVETVKRLQRIVRDPRAGYEQSLSVLKHAKVSKKGMITKTSIMLGLGETDDEVKEAMTDLRAIDVDILTFGQYLQPTPLHLTVKEYVSPEKFAYWKEYGESIGFRYVASGPLVRSSYRAGELFVKTMVKESAKEAAAIS

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A2K1WSB4A0A2K1WSB4_POPTRLIP1P397

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias49-71Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM009308
EMBL· GenBank· DDBJ
ERP49380.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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