B9LS20 · GUOP_HALLT

Function

function

Phosphorylase involved in the non-carboxylating pentose bisphosphate pathway, a nucleoside degradation pathway present in some halophilic archaea (PubMed:36434094).
Catalyzes the phosphorolytic cleavage of guanosine to guanine and ribose-1-phosphate (R1P) (PubMed:36434094).
Exhibits the highest activity toward guanosine, but also shows lower activity against inosine and adenosine (PubMed:36434094).

Catalytic activity

Activity regulation

Activity is higher at low KCl concentrations.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
56 μMguanosine
4.8 mMphosphate
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
1.5 μmol/min/mgtoward guanosine
2.1 μmol/min/mgtoward phosphate

pH Dependence

Optimum pH is 7.5.

Temperature Dependence

Optimum temperature is 30 degrees Celsius.

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionguanosine phosphorylase activity
Molecular FunctionS-methyl-5-thioadenosine phosphorylase activity
Biological Processnucleoside catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Guanosine phosphorylase
  • EC number

Gene names

    • Ordered locus names
      Hlac_2318

Organism names

Accessions

  • Primary accession
    B9LS20

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004597121-241Guanosine phosphorylase

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the PNP/UDP phosphorylase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    241
  • Mass (Da)
    25,738
  • Last updated
    2009-03-24 v1
  • Checksum
    437DEF34EF9D5A24
MAKQPHLLVEEGDVHEIAIIPGDPGRVDRIADLCDDSELVAQNREYKIVNASYDGTDLTICSTGIGCPSAAIAVEELSRVGVETFLRCGTCGALQADMEVGDMVVATGAAKEEGTSKRYESVEYPAVPDYDALTELVGAAEDNDEEIHVGPIVSDDAFYNESDEYVDDWNDANLLAIEMEAATVFALARRKGLRAGAICTVDGNLVAGNQKGADSDDELPEKAKDNVERAIRITLNAVTAL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001365
EMBL· GenBank· DDBJ
ACM57894.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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