B8ZTI5 · PCKG_MYCLB
- ProteinPhosphoenolpyruvate carboxykinase [GTP]
- GenepckG
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids609 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Catalytic activity
- GTP + oxaloacetate = CO2 + GDP + phosphoenolpyruvate
Cofactor
Note: Binds 1 Mn2+ ion per subunit.
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 81 | substrate | ||||
Sequence: R | ||||||
Binding site | 220-222 | substrate | ||||
Sequence: YGG | ||||||
Binding site | 229 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 249 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 271 | substrate | ||||
Sequence: S | ||||||
Binding site | 272-277 | GTP (UniProtKB | ChEBI) | ||||
Sequence: ACGKTN | ||||||
Active site | 273 | |||||
Sequence: C | ||||||
Binding site | 296 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 387-389 | substrate | ||||
Sequence: NSR | ||||||
Binding site | 389 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 420 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 515-518 | GTP (UniProtKB | ChEBI) | ||||
Sequence: FGEN |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | GTP binding | |
Molecular Function | manganese ion binding | |
Molecular Function | phosphoenolpyruvate carboxykinase (GTP) activity | |
Biological Process | gluconeogenesis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoenolpyruvate carboxykinase [GTP]
- EC number
- Short namesPEP carboxykinase ; PEPCK
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium
Accessions
- Primary accessionB8ZTI5
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000192345 | 1-609 | Phosphoenolpyruvate carboxykinase [GTP] | |||
Sequence: MTSATIPGLDTAPTNHQKLLSWLEEVAELTHPAQVVFADGSDEEWQQLTERLVAAGTFKKLNDEKHPNSYLALSDPSDVARVESRTFICSEREIDAGPTNNWMDPAEMRSTMTELYRGCMRGRTMWVVPFCMGPPGAEDPKLGVEITDSEYVVASMKVMTRMGTSALEKIGDDGFFVKALHSVGAPLQPGQQDMPWPCNETKYITHFPETREIWSYGSGYGGNALLGKKCYSLRIASAMAHDEGWLAEHMLILKLISPENKAYYFAAAFPSACGKTNLAMLQPTIPGWRAETLGDDIAWMRFGKDGRLYAVNPEFGFFGVAPGTNWKSNPNAMRTIAAGNTVFTNVALTDDNDVWWEGLEGEPAHLIDWKGNDWYAGKTETPAAHSNSRYCTPMSQCPILAPEWDDPRGVPISVILFGARRKTTVPLVTQARNWQHGVFMGATMGSEQTAAAEGKVGTVRRDPMAMLPFMGYHVGDYFQHWIDLGKHSDESNLPKVFFVNWFRRGEGGKFLWPGFGENSRVLKWIVDRIEHKAGGQDTLIGIVPTATDLDLDGLDISSDDVAKALAVDPAEWRNELPLIEEWFEFVGDKLPSGMQDEFEALKQRLAKED |
Interaction
Subunit
Monomer.
Structure
Sequence
- Sequence statusComplete
- Length609
- Mass (Da)67,732
- Last updated2009-03-03 v1
- Checksum8916B6733622590B
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FM211192 EMBL· GenBank· DDBJ | CAR72724.1 EMBL· GenBank· DDBJ | Genomic DNA |