B8ZQK0 · LIPB_MYCLB
- ProteinOctanoyltransferase
- GenelipB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids235 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.
Miscellaneous
In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes.
Catalytic activity
- octanoyl-[ACP] + L-lysyl-[protein] = N6-octanoyl-L-lysyl-[protein] + holo-[ACP] + H+
Pathway
Protein modification; protein lipoylation via endogenous pathway; protein N6-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 78-85 | substrate | |||
Site | 147 | Lowers pKa of active site Cys | |||
Binding site | 150-152 | substrate | |||
Binding site | 163-165 | substrate | |||
Active site | 181 | Acyl-thioester intermediate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | lipoyl(octanoyl) transferase activity | |
Biological Process | protein lipoylation |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameOctanoyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium
Accessions
- Primary accessionB8ZQK0
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000116550 | 1-235 | Octanoyltransferase | ||
Structure
Sequence
- Sequence statusComplete
- Length235
- Mass (Da)25,002
- Last updated2009-03-03 v1
- MD5 ChecksumEFB3138B4694ED35CD43B1B8BB4FF400
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FM211192 EMBL· GenBank· DDBJ | CAR70954.1 EMBL· GenBank· DDBJ | Genomic DNA |