B8YB65 · RPO13_SACSH
- ProteinDNA-directed RNA polymerase subunit Rpo13
- Generpo13
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids104 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (Probable). A molten-globule protein, it binds dsDNA in the RNAP, in vitro binds dsDNA but not ssDNA (Probable) (PubMed:22848102).
Its position in RNAP implies it functions in both transcription initiation and elongation (Probable)
Its position in RNAP implies it functions in both transcription initiation and elongation (Probable)
Catalytic activity
- RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 32 | DNA (UniProtKB | ChEBI); template DNA | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | nucleotidyltransferase activity | |
Molecular Function | RNA polymerase binding |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit Rpo13
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Sulfolobales > Sulfolobaceae > Saccharolobus
Accessions
- Primary accessionB8YB65
- Secondary accessions
Proteomes
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 1-33 | Decreased stability, still binds DNA. | |||
Mutagenesis | 81-104 | Protein is stable, no longer binds DNA. | |||
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000453788 | 1-104 | DNA-directed RNA polymerase subunit Rpo13 | ||
Interaction
Subunit
Part of the 13-subunit RNA polymerase complex. Rpo1N and Rpo5 form a cleft which docks Rpo13 (PubMed:19419240, PubMed:21265742, PubMed:22848102).
Forms predominantly dimers in solution, although monomers and trimers can also be seen (PubMed:21265742, PubMed:22848102).
Found associated with RNAP but also as a homodimer pool in the cytoplasm in vivo (PubMed:22848102).
Forms predominantly dimers in solution, although monomers and trimers can also be seen (PubMed:21265742, PubMed:22848102).
Found associated with RNAP but also as a homodimer pool in the cytoplasm in vivo (PubMed:22848102).
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-34 | Disordered | |||
Compositional bias | 11-28 | Acidic residues | |||
Region | 76-104 | Disordered | |||
Region | 81-104 | Required to bind DNA | |||
Compositional bias | 83-97 | Basic residues | |||
Domain
Forms a helix-turn-helix motif with helix 1 (residues 38-56) nearly parallel to helix 2 (residues 61-82); helix 1 contacts both Rpo5 and Rpo1N. The C-terminal region (residues 81-104) is required for DNA-binding.
Sequence similarities
Belongs to the archaeal Rpo13 RNA polymerase subunit family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length104
- Mass (Da)12,148
- Last updated2009-03-03 v1
- MD5 Checksum691466EED6990FEA51C61EF1D30D59EC
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 11-28 | Acidic residues | |||
Compositional bias | 83-97 | Basic residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FJ515677 EMBL· GenBank· DDBJ | ACL36500.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP077717 EMBL· GenBank· DDBJ | QXJ30148.1 EMBL· GenBank· DDBJ | Genomic DNA |