B8Y445 · RAMA_METBA
- Protein[Co(II) methylated amine-specific corrinoid protein] reductase
- GeneramA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids540 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Reductase required for the activation of corrinoid-dependent methylamine methyltransferase reactions during methanogenesis (PubMed:19043046).
Mediates the ATP-dependent reduction of corrinoid proteins from the inactive cobalt(II) state to the active cobalt(I) state (PubMed:19043046).
Acts on the corrinoid proteins involved in methanogenesis from monomethylamine (MMA), dimethylamine (DMA) and trimethylamine (TMA), namely MtmC, MtbC and MttC, respectively (PubMed:19043046).
Mediates the ATP-dependent reduction of corrinoid proteins from the inactive cobalt(II) state to the active cobalt(I) state (PubMed:19043046).
Acts on the corrinoid proteins involved in methanogenesis from monomethylamine (MMA), dimethylamine (DMA) and trimethylamine (TMA), namely MtmC, MtbC and MttC, respectively (PubMed:19043046).
Catalytic activity
- 2 Co(II)-[methylamine-specific corrinoid protein] + AH2 + ATP + H2O = 2 Co(I)-[methylamine-specific corrinoid protein] + A + ADP + phosphate + 3 H+This reaction proceeds in the forward direction.
Cofactor
Note: Binds 2 [4Fe-4S] clusters.
Pathway
One-carbon metabolism; methanogenesis from methylamine.
One-carbon metabolism; methanogenesis from dimethylamine.
One-carbon metabolism; methanogenesis from trimethylamine.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 480 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 483 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 486 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 490 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | |||
Binding site | 513 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | |||
Binding site | 518 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | |||
Binding site | 521 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | |||
Binding site | 525 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | oxidoreductase activity | |
Biological Process | methanogenesis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name[Co(II) methylated amine-specific corrinoid protein] reductase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Stenosarchaea group > Methanomicrobia > Methanosarcinales > Methanosarcinaceae > Methanosarcina
Accessions
- Primary accessionB8Y445
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000460048 | 1-540 | [Co(II) methylated amine-specific corrinoid protein] reductase | ||
Interaction
Subunit
Monomer.
Structure
Sequence
- Sequence statusComplete
- Length540
- Mass (Da)59,084
- Last updated2009-03-03 v1
- MD5 Checksum2194C25B8C3A12AD57D61C4E7333BD5F
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FJ477063 EMBL· GenBank· DDBJ | ACL01114.1 EMBL· GenBank· DDBJ | Genomic DNA |