B8Y445 · RAMA_METBA

  • Protein
    [Co(II) methylated amine-specific corrinoid protein] reductase
  • Gene
    ramA
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Reductase required for the activation of corrinoid-dependent methylamine methyltransferase reactions during methanogenesis (PubMed:19043046).
Mediates the ATP-dependent reduction of corrinoid proteins from the inactive cobalt(II) state to the active cobalt(I) state (PubMed:19043046).
Acts on the corrinoid proteins involved in methanogenesis from monomethylamine (MMA), dimethylamine (DMA) and trimethylamine (TMA), namely MtmC, MtbC and MttC, respectively (PubMed:19043046).

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 2 [4Fe-4S] clusters.

Pathway

One-carbon metabolism; methanogenesis from methylamine.
One-carbon metabolism; methanogenesis from dimethylamine.
One-carbon metabolism; methanogenesis from trimethylamine.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site480[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site483[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site486[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site490[4Fe-4S] cluster 2 (UniProtKB | ChEBI)
Binding site513[4Fe-4S] cluster 2 (UniProtKB | ChEBI)
Binding site518[4Fe-4S] cluster 2 (UniProtKB | ChEBI)
Binding site521[4Fe-4S] cluster 2 (UniProtKB | ChEBI)
Binding site525[4Fe-4S] cluster 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular Functionoxidoreductase activity
Biological Processmethanogenesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    [Co(II) methylated amine-specific corrinoid protein] reductase
  • EC number
  • Alternative names
    • Corrinoid activation enzyme RamA

Gene names

    • Name
      ramA

Organism names

  • Taxonomic identifier
  • Strain
    • ATCC 43569 / MS / DSM 800 / JCM 10043 / NBRC 100474
  • Taxonomic lineage
    Archaea > Euryarchaeota > Stenosarchaea group > Methanomicrobia > Methanosarcinales > Methanosarcinaceae > Methanosarcina

Accessions

  • Primary accession
    B8Y445

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004600481-540[Co(II) methylated amine-specific corrinoid protein] reductase

Interaction

Subunit

Monomer.

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain471-5004Fe-4S ferredoxin-type 1
Domain504-5354Fe-4S ferredoxin-type 2

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    540
  • Mass (Da)
    59,084
  • Last updated
    2009-03-03 v1
  • MD5 Checksum
    2194C25B8C3A12AD57D61C4E7333BD5F
MYGIALDLGTSGFRTQLIDLETKETLKTVITMGHPLPGGNVMDHLDFAITTGEDVAHEVIIETIRRMFLQFDIDLSRVERLAVCGNPIQLSLFQNTEIRDLAYAGENKQKMLGVRNVKRDARVFPASEIFGEKYLSNCEIIVPPAIKHEIGADALAMMLETDFLIQPEPSLVTDYGTNAEMALKIGDRIITASAAAGPAIEGQGISSGMLASPGAICDVKPEGQYWRIIVLDREMEKQDAYLIDPVKGEIKDSYGFEAVGITGTGVISAFAMALKGGLIEKFPKLPNGKLILGPGIEITEKDVEEAGKAIGAIRAAHMTLIVESGIKYEDLEYAYMSGASGAYVDAEDARRLGAAPGYAKKIVQFGNTSLALARELVLEKSRLDDVIEIAKKITADHLMMATSETFNNFYLCELSYWTQGMPLETYDQMLELYGLPPLPKILEHVTIEKRVSKDIEEVGSGGLSILKEIGIILEVPVEKCVYCKKCVKECPEAALEIVERDGQRIAKYDSQKCLGTSCRRCVGVCPEDAIDITKLKITAK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FJ477063
EMBL· GenBank· DDBJ
ACL01114.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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