B8XA40 · ZFPS_SOLHA
- Protein(2Z,6Z)-farnesyl diphosphate synthase, chloroplastic
- GeneZFPS
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids303 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Specifically forms (2Z,6Z)-farnesyl diphosphate (Z,Z-FPP) from dimethylallyl diphosphate (DMAPP) and isopentenyl diphosphate (IPP). Also able to use nerylpyrophosphate (NPP) as substrate. Involved in the biosynthesis of several sesquiterpenes.
Catalytic activity
- dimethylallyl diphosphate + 2 isopentenyl diphosphate = (2Z,6Z)-farnesyl diphosphate + 2 diphosphate
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
10 μM | dimethylallyl diphosphate in the presence of 35 uM isopentenyl diphosphate | |||||
35 μM | dimethylallyl diphosphate in the presence of 210 uM isopentenyl diphosphate | |||||
10 μM | nerylpyrophosphate in the presence of 180 uM isopentenyl diphosphate |
isopentenyl diphosphate acts as activator of its own catalysis.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 86 | |||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast stroma | |
Molecular Function | 2-cis,6-cis-farnesyl pyrophosphate synthase activity | |
Molecular Function | dehydrodolichyl diphosphate synthase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | polyprenyltransferase activity | |
Biological Process | plastid membrane organization | |
Biological Process | polyprenol biosynthetic process | |
Biological Process | response to cold |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name(2Z,6Z)-farnesyl diphosphate synthase, chloroplastic
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Solanoideae > Solaneae > Solanum > Solanum subgen. Lycopersicon
Accessions
- Primary accessionB8XA40
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-45 | Chloroplast | ||||
Sequence: MSSLVLQCWKLSSPSLILQQNTSISMGAFKGIHKLQIPNSPLTVS | ||||||
Chain | PRO_0000405119 | 46-303 | (2Z,6Z)-farnesyl diphosphate synthase, chloroplastic | |||
Sequence: ARGLNKISCSLSLQTEKLCYEDNDNDLDEELMPKHIALIMDGNRRWAKDKGLDVSEGHKHLFPKLKEICDISSKLGIQVITAFAFSTENWKRAKGEVDFLMQMFEELYDEFSRSGVRVSIIGCKTDLPMTLQKCIALTEETTKGNKGLHLVIALNYGGYYDILQATKSIVNKAMNGLLDVEDINKNLFDQELESKCPNPDLLIRTGGDQRVSNFLLWQLAYTEFYFTKTLFPDFGEEDLKEAIINFQQRHRRFGGHTY |
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length303
- Mass (Da)34,430
- Last updated2009-03-03 v1
- ChecksumC9289E0BC89193BE
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FJ194969 EMBL· GenBank· DDBJ | ACJ38408.1 EMBL· GenBank· DDBJ | mRNA |