B8QSF0 · B8QSF0_9GEMI

Function

function

Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the + strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities.

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Divalent metal cations, possibly Mg2+ or Mn2+.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site49a divalent metal cation (UniProtKB | ChEBI)
Binding site57a divalent metal cation (UniProtKB | ChEBI)
Binding site59a divalent metal cation (UniProtKB | ChEBI)
Active site103For DNA cleavage activity

GO annotations

AspectTerm
Cellular Componenthost cell nucleus
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular Functionendodeoxyribonuclease activity, producing 5'-phosphomonoesters
Molecular Functionhelicase activity
Molecular Functionmetal ion binding
Molecular Functionnucleotidyltransferase activity
Molecular Functionstructural molecule activity
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Replication-associated protein
  • EC number
  • Short names
    Rep

Gene names

    • Name
      AC1

Organism names

Accessions

  • Primary accession
    B8QSF0

Subcellular Location

Keywords

PTM/Processing

Keywords

Interaction

Subunit

Homooligomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain8-116CRESS-DNA virus Rep endonuclease

Domain

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Sequence similarities

Belongs to the geminiviridae Rep protein family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    352
  • Mass (Da)
    39,899
  • Last updated
    2009-03-03 v1
  • Checksum
    6EFCA2356BBC103E
MPRAGRFQINAKNYFITYPRCSLAKEEALSQLKAISYPTNIKFIRVCRELHQDGVPHLHVLIQFEGKFQCTNPRFFDLISPSRSTHFHPNIQGAKSSSDVKAYIEKGGEFIDDGIFQVDARSARGEGQHLAQVYAEALNASSKSEALQIIKEKDPKSFFLQFHNISANADRIFQAPPQTYVSPFLSSSFTQVPEDIEVWVSENICRPAARPWRPISIVLEGDSRTGKTMWARSLGPHNYLCGHLDLSPKIYSNDAWYNVIDDVDPHYLKHFKEFMGAQRDWQSNTKYGKPIQIKGGIPTIFLCNPGPNSSYKEYLDEDKNSNLKNWAIKNALFISLTEPLFSSTDQSQAQAS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
EU685323
EMBL· GenBank· DDBJ
ACF42159.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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