B8QHP5 · CP52N_STABO
- ProteinCytochrome P450 52-N1
- Genecyp52N1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids523 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the terminal (at the omega-position) hydroxylation of a fatty acid. Probably involved in alkane metabolism. Linoleic acid is the preferred substrate, but it acts on various other C-16, C-18 and C-20 saturated and unsaturated fatty acids, namely palmitic, palmitoleic, stearic, oleic, alpha-linoleic, arachidonic and myristic acid.
Catalytic activity
- an omega-methyl-long-chain fatty acid + reduced [NADPH--hemoprotein reductase] + O2 = an omega-hydroxy-long-chain fatty acid + oxidized [NADPH--hemoprotein reductase] + H2O + H+
- (9Z,12Z)-octadecadienoate + reduced [NADPH--hemoprotein reductase] + O2 = 18-hydroxy-(9Z,12Z)-octadecadienoate + oxidized [NADPH--hemoprotein reductase] + H2O + H+
- (9Z)-hexadecenoate + reduced [NADPH--hemoprotein reductase] + O2 = (9Z)-16-hydroxyhexadec-9-enoate + oxidized [NADPH--hemoprotein reductase] + H2O + H+
Cofactor
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 469 | Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | long-chain fatty acid omega-hydroxylase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytochrome P450 52-N1
- EC number
- Short namesCYP52-N1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Trichomonascaceae > Starmerella
Accessions
- Primary accessionB8QHP5
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 5-25 | Helical | |||
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000447697 | 1-523 | Cytochrome P450 52-N1 | ||
Expression
Induction
Up-regulated when grown on alkanes as sole carbon source.
Structure
Sequence
- Sequence statusComplete
- Length523
- Mass (Da)59,596
- Last updated2009-03-03 v1
- Checksum1987EDDCA7A35705
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
EU552421 EMBL· GenBank· DDBJ | ACD75402.1 EMBL· GenBank· DDBJ | Genomic DNA |