B8J7G1 · PCKG_ANAD2
- ProteinPhosphoenolpyruvate carboxykinase [GTP]
- GenepckG
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids596 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Catalytic activity
- GTP + oxaloacetate = CO2 + GDP + phosphoenolpyruvate
Cofactor
Note: Binds 1 Mn2+ ion per subunit.
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 77 | substrate | ||||
Sequence: R | ||||||
Binding site | 205-207 | substrate | ||||
Sequence: YGG | ||||||
Binding site | 214 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 234 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 256 | substrate | ||||
Sequence: S | ||||||
Binding site | 257-262 | GTP (UniProtKB | ChEBI) | ||||
Sequence: ACGKTN | ||||||
Active site | 258 | |||||
Sequence: C | ||||||
Binding site | 283 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 373-375 | substrate | ||||
Sequence: NSR | ||||||
Binding site | 375 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 406 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 499-502 | GTP (UniProtKB | ChEBI) | ||||
Sequence: YGDN |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | GTP binding | |
Molecular Function | manganese ion binding | |
Molecular Function | phosphoenolpyruvate carboxykinase (GTP) activity | |
Biological Process | cellular response to glucose stimulus | |
Biological Process | gluconeogenesis | |
Biological Process | glycerol biosynthetic process from pyruvate | |
Biological Process | propionate catabolic process | |
Biological Process | response to lipid | |
Biological Process | response to starvation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoenolpyruvate carboxykinase [GTP]
- EC number
- Short namesPEP carboxykinase ; PEPCK
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Myxococcota > Myxococcia > Myxococcales > Cystobacterineae > Anaeromyxobacteraceae > Anaeromyxobacter
Accessions
- Primary accessionB8J7G1
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000192343 | 1-596 | Phosphoenolpyruvate carboxykinase [GTP] | |||
Sequence: MSTSPAARPTSNAHLLGWVDEMAKLCKPDRVYWCDGSEAEKKRLTDDAVAAKVLIPLDQQKWPGCHYHHSNSSDVARVEHLTFICTPTKEQAGPTNNWMEPKEAYRKLGAIFDGSMKGRTMYVVPYVMGPSTSPFAKVGIEITDSVYVALNMGIMARMGKVALDRLGDSDEFNRGLHSVADCNPERRFICHFPQDNTIWSVGSGYGGNALLGKKCLALRIASYLAKNEGWLAEHMLILEAESPTGEKQYVAAAFPSACGKTNFAMMIPPAAFPGWKIRTVGDDISWMRVGEDGRLWAVNPENGYFGVAPGTNRKTNPNAMDSVRKDTIFTNVARTPDGDIWWEGMDHEAPAELIDWKGQPWKKGSTEKAAHPNSRFTAPAKNNPAISPLVDDPKGVPISAIIFGGRRSTTVPLVLEAFNWTHGVYLGSTMGSETTAAATGQVGVVRRDPMAMLPFIGYDCGSYLQHWLDMQSRIPNPPKIFLVNWFRKSAEGKFLWPGYGDNMRVLKWMLDRAAGRAPAKETLLGYTPGDSGLDLHGLDVSKDAIAAATQIDLGEWEQELESQSEWFEKLGKTLPRPLALQRELLLERVRAARKVK |
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 362-388 | Disordered | ||||
Sequence: KKGSTEKAAHPNSRFTAPAKNNPAISP |
Sequence similarities
Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length596
- Mass (Da)65,869
- Last updated2009-03-03 v1
- Checksum053D58D526ECC4A9
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001359 EMBL· GenBank· DDBJ | ACL67141.1 EMBL· GenBank· DDBJ | Genomic DNA |