B8I6E1 · ISPG_RUMCH
- Protein4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
- GeneispG
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids353 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.
Catalytic activity
- (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + oxidized [flavodoxin] + H2O + 2 H+ = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin]
Cofactor
Note: Binds 1 [4Fe-4S] cluster.
Pathway
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 5/6.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 268 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 271 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 303 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 310 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity (ferredoxin) | |
Molecular Function | 4-hydroxy-3-methylbut-2-enyl-diphosphate synthase activity (flavodoxin) | |
Molecular Function | iron ion binding | |
Biological Process | isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway | |
Biological Process | terpenoid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Oscillospiraceae > Ruminiclostridium
Accessions
- Primary accessionB8I6E1
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000123440 | 1-353 | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) | ||
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length353
- Mass (Da)37,931
- Last updated2009-03-03 v1
- MD5 Checksum98FC843B2C39AD2C28E42C50DBDE7EE7
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001348 EMBL· GenBank· DDBJ | ACL74833.1 EMBL· GenBank· DDBJ | Genomic DNA |