B8AJL3 · C90B2_ORYSI
- ProteinSteroid (22S)-hydroxylase
- GeneCYP90B2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids506 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the C22-alpha-hydroxylation step in brassinosteroid biosynthesis, which is the rate-limiting step in this biosynthetic pathway (By similarity).
Catalyzes the conversion of campesterol (CR) to (22S)-22-hydroxycampesterol (22-OHCR, 22-hydroxyCR) and of campestanol (CN) to 6-deoxocathasterone (6-deoxoCT) (By similarity).
Catalyzes the conversion of campesterol (CR) to (22S)-22-hydroxycampesterol (22-OHCR, 22-hydroxyCR) and of campestanol (CN) to 6-deoxocathasterone (6-deoxoCT) (By similarity).
Catalytic activity
- a C28-steroid + O2 + reduced [NADPH--hemoprotein reductase] = a (22S)-22-hydroxy C28-steroid + H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- campesterol + O2 + reduced [NADPH--hemoprotein reductase] = (22S)-22-hydroxycampesterol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- campestanol + O2 + reduced [NADPH--hemoprotein reductase] = 6-deoxycathasterone + H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
Cofactor
Pathway
Plant hormone biosynthesis; brassinosteroid biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | membrane | |
Molecular Function | fatty acid alpha-hydroxylase activity | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | |
Molecular Function | steroid 22-alpha hydroxylase activity | |
Biological Process | brassinosteroid biosynthetic process | |
Biological Process | jasmonic acid mediated signaling pathway | |
Biological Process | leaf shaping | |
Biological Process | response to brassinosteroid | |
Biological Process | unidimensional cell growth |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSteroid (22S)-hydroxylase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionB8AJL3
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 12-32 | Helical | ||||
Sequence: LLFFLPFILLALLTFYTTTVA |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_5002867622 | 1-506 | Steroid (22S)-hydroxylase | |||
Sequence: MAAMMASITSELLFFLPFILLALLTFYTTTVAKCHGGHWWRGGTTPAKRKRMNLPPGAAGWPLVGETFGYLRAHPATSVGRFMEQHIARYGKIYRSSLFGERTVVSADAGLNRYILQNEGRLFECSYPRSIGGILGKWSMLVLVGDPHREMRAISLNFLSSVRLRAVLLPEVERHTLLVLRAWLPSSTFSAQHQAKKFTFNLMAKNIMSMDPGEEETERLRREYITFMKGVVSAPLNLPGTPYWKALKSRAAILGVIERKMEERVEKLSKEDASVEQDDLLGWALKQSNLSKEQILDLLLSLLFAGHETSSMALALAIFFLEGCPKAVQELREEHLGIARRQRLRGECKLSWEDYKEMVFTQCVINETLRLGNVVRFLHRKVIKDVHYKGYDIPSGWKILPVLAAVHLDSSLYEDPQRFNPWRWKSSGSSGGLAQSSSFMPYGGGTRLCAGSELAKLEMAVFLHHLVLNFRWELAEPDQAFVFPFVDFPKGLPIRVHRIAQDDEQE |
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length506
- Mass (Da)57,345
- Last updated2009-03-03 v1
- Checksum39A855B2C11125B9
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CM000128 EMBL· GenBank· DDBJ | EEC74804.1 EMBL· GenBank· DDBJ | Genomic DNA |