B8AJL3 · C90B2_ORYSI

Function

function

Catalyzes the C22-alpha-hydroxylation step in brassinosteroid biosynthesis, which is the rate-limiting step in this biosynthetic pathway (By similarity).
Catalyzes the conversion of campesterol (CR) to (22S)-22-hydroxycampesterol (22-OHCR, 22-hydroxyCR) and of campestanol (CN) to 6-deoxocathasterone (6-deoxoCT) (By similarity).

Catalytic activity

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Pathway

Plant hormone biosynthesis; brassinosteroid biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site449Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum
Cellular Componentmembrane
Molecular Functionfatty acid alpha-hydroxylase activity
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Molecular Functionsteroid 22-alpha hydroxylase activity
Biological Processbrassinosteroid biosynthetic process
Biological Processjasmonic acid mediated signaling pathway
Biological Processleaf shaping
Biological Processresponse to brassinosteroid
Biological Processunidimensional cell growth

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Steroid (22S)-hydroxylase
  • EC number
  • Alternative names
    • (22S)-22-hydroxycampesterol synthase
    • 6-deoxocathasterone synthase
    • Cytochrome P450 90B2
      (OsCYP90B2
      )

Gene names

    • Name
      CYP90B2
    • ORF names
      OsI_10612

Organism names

  • Taxonomic identifier
  • Strain
    • cv. 93-11
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa

Accessions

  • Primary accession
    B8AJL3

Proteomes

Genome annotation databases

Subcellular Location

Membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane12-32Helical

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_50028676221-506Steroid (22S)-hydroxylase

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    506
  • Mass (Da)
    57,345
  • Last updated
    2009-03-03 v1
  • Checksum
    39A855B2C11125B9
MAAMMASITSELLFFLPFILLALLTFYTTTVAKCHGGHWWRGGTTPAKRKRMNLPPGAAGWPLVGETFGYLRAHPATSVGRFMEQHIARYGKIYRSSLFGERTVVSADAGLNRYILQNEGRLFECSYPRSIGGILGKWSMLVLVGDPHREMRAISLNFLSSVRLRAVLLPEVERHTLLVLRAWLPSSTFSAQHQAKKFTFNLMAKNIMSMDPGEEETERLRREYITFMKGVVSAPLNLPGTPYWKALKSRAAILGVIERKMEERVEKLSKEDASVEQDDLLGWALKQSNLSKEQILDLLLSLLFAGHETSSMALALAIFFLEGCPKAVQELREEHLGIARRQRLRGECKLSWEDYKEMVFTQCVINETLRLGNVVRFLHRKVIKDVHYKGYDIPSGWKILPVLAAVHLDSSLYEDPQRFNPWRWKSSGSSGGLAQSSSFMPYGGGTRLCAGSELAKLEMAVFLHHLVLNFRWELAEPDQAFVFPFVDFPKGLPIRVHRIAQDDEQE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM000128
EMBL· GenBank· DDBJ
EEC74804.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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