B7Z7Q4 · B7Z7Q4_HUMAN
- ProteinProtein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids638 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Participates in O-mannosyl glycosylation by catalyzing the addition of N-acetylglucosamine to O-linked mannose on glycoproteins. Catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins, providing the necessary basis for the addition of further carbohydrate moieties. Is specific for alpha linked terminal mannose.
Catalytic activity
- 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl)-L-threonyl-[protein] + UDP + H+
Cofactor
Note: The manganese ion interacts primarily with the substrate UDP-N-acetylglucosamine.
Pathway
Protein modification; protein glycosylation.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Golgi membrane | |
Molecular Function | acetylglucosaminyltransferase activity | |
Molecular Function | carbohydrate binding | |
Molecular Function | manganese ion binding | |
Biological Process | protein glycosylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase
- EC number
- Short namesPOMGnT1
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionB7Z7Q4
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Single-pass type II membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 20-37 | Helical | ||||
Sequence: GAVLFLLVTVIVNIKLIL |
Keywords
- Cellular component
Disease & Variants
Organism-specific databases
PTM/Processing
Interaction
Subunit
Interacts with DAG1 (via O-linked mannose moiety). Interacts (via transmembrane domain) with FKTN; the interaction is direct and is required for normal location in Golgi membranes.
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 46-74 | Disordered | ||||
Sequence: ANEDPEPEQDYDEALGRLEPPRRRGSGPR | ||||||
Compositional bias | 56-74 | Basic and acidic residues | ||||
Sequence: YDEALGRLEPPRRRGSGPR | ||||||
Domain | 107-196 | ILEI/PANDER | ||||
Sequence: RGIHVIVLNQATGHVMAKRVFDTYSPHEDEAMVLFLNMVAPGRVLICTVKDEGSFHLKDTAKALLRSLGSQAGPALGWRDTWAFVGRKGG | ||||||
Region | 612-638 | Disordered | ||||
Sequence: KKPPSVTPIFLEPPPKEEGAPGAPEQT |
Domain
The stem domain mediates specific interaction with beta-linked N-acetylglucosamine moieties of O-glycosylated proteins. It also interacts with its product, N-acetyl-beta-D-glucosaminyl-(1->2)-O-alpha-D-mannosylprotein.
Sequence similarities
Belongs to the glycosyltransferase 13 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length638
- Mass (Da)72,447
- Last updated2009-03-03 v1
- Checksum6B9D4E06B80E7116
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 56-74 | Basic and acidic residues | ||||
Sequence: YDEALGRLEPPRRRGSGPR |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK302379 EMBL· GenBank· DDBJ | BAH13690.1 EMBL· GenBank· DDBJ | mRNA |