B7YZV4 · PDE1_DROME
- ProteinDual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1
- GenePde1c
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids834 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Cyclic nucleotide phosphodiesterase with a dual specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes (PubMed:15673286).
Required for male fertility and male mating behavior (PubMed:20551439).
Required for male fertility and male mating behavior (PubMed:20551439).
Catalytic activity
- a nucleoside 3',5'-cyclic phosphate + H2O = a nucleoside 5'-phosphate + H+This reaction proceeds in the forward direction.
- 3',5'-cyclic GMP + H2O = GMP + H+This reaction proceeds in the forward direction.
- 3',5'-cyclic AMP + H2O = AMP + H+This reaction proceeds in the forward direction.
Cofactor
Protein has several cofactor binding sites:
Note: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions.
Note: Binds 2 divalent metal cations per subunit. Site 2 has a preference for magnesium ions.
Activity regulation
Type I PDE are activated by the binding of calmodulin in the presence of Ca2+ (PubMed:15673286).
Inhibited by zaprinast and sildenafil (PubMed:15673286).
Inhibited by zaprinast and sildenafil (PubMed:15673286).
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
15.3 μM | 3',5'-cyclic GMP | |||||
20.5 μM | 3',5'-cyclic AMP |
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 469 | Proton donor | |||
Binding site | 473 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 509 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 510 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 510 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 617 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular space | |
Molecular Function | 3',5'-cGMP-inhibited cyclic-nucleotide phosphodiesterase activity | |
Molecular Function | 3',5'-cGMP-stimulated cyclic-nucleotide phosphodiesterase activity | |
Molecular Function | 3',5'-cyclic-AMP phosphodiesterase activity | |
Molecular Function | 3',5'-cyclic-GMP phosphodiesterase activity | |
Molecular Function | calmodulin binding | |
Molecular Function | calmodulin-activated 3',5'-cyclic-GMP phosphodiesterase activity | |
Molecular Function | calmodulin-activated dual specificity 3',5'-cyclic-GMP, 3',5'-cyclic-AMP phosphodiesterase activity | |
Molecular Function | metal ion binding | |
Biological Process | cAMP metabolic process | |
Biological Process | cAMP-mediated signaling | |
Biological Process | cGMP metabolic process | |
Biological Process | male mating behavior | |
Biological Process | sexual reproduction |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1
- EC number
- Short namesPDE1
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionB7YZV4
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000438861 | 1-834 | Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1 | ||
Proteomic databases
Expression
Tissue specificity
Expressed in the head (at protein level) (PubMed:15673286).
Expressed in Malpighian tubules (PubMed:15673286).
Expressed in neurons in the brain and ventral ganglia with male flies having higher levels of expression in the abdominal ganglia compared to female flies (PubMed:20551439).
Expressed in Malpighian tubules (PubMed:15673286).
Expressed in neurons in the brain and ventral ganglia with male flies having higher levels of expression in the abdominal ganglia compared to female flies (PubMed:20551439).
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 152-338 | Disordered | |||
Compositional bias | 189-226 | Polar residues | |||
Compositional bias | 258-300 | Polar residues | |||
Region | 364-387 | Calmodulin-binding | |||
Domain | 392-786 | PDEase | |||
Region | 720-744 | Disordered | |||
Region | 797-834 | Disordered | |||
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
B7YZV4-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameB
- SynonymsG
- Length834
- Mass (Da)91,455
- Last updated2009-03-03 v1
- ChecksumD027A9481010C665
B7YZV4-2
- NameC
- Differences from canonical
- 1-322: MQPSSPNATNYLADNIQISSANLSQTEMVVGRDSADYTAMHSINVGVGNSFLRGDTDIPQESGHSFETPSNMSFTAGQWDTESLPPVDTPDALNKAAGRIRSLLRRMDHETVAYEDMQRNLHYAARVLEAVFIDESREGCNGNCKNLNCSRHSHGRDDQQQDNNNSNRSCSLQEASPGGAGAGVTPGADNQDSIESRTKGVSQAPQTHSGPTGPPSNTSSETIAQPAPKLQPALETVRESVMEESPSKDPGDKGPPPPASTSTLTSQTTTSSSATAEPSAKAAESQAGSAGSSGSCSNPAAVHRQRRLRTPTWARSMSTNKT → MYEPGTSSEEGVVSETEPEPAGVSVSVSVSVSEEPSASRSSRLTVITVVVVVVAVRAMSRLRRESSRQSVITVELIQFIIQYRAKETQEKKKR
Computationally mapped potential isoform sequences
There are 6 potential isoforms mapped to this entry
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Alternative sequence | VSP_058752 | 1-322 | in isoform C | ||
Compositional bias | 189-226 | Polar residues | |||
Compositional bias | 258-300 | Polar residues | |||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE014134 EMBL· GenBank· DDBJ | AAF53123.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014134 EMBL· GenBank· DDBJ | ACL83022.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014134 EMBL· GenBank· DDBJ | ACL83026.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY118396 EMBL· GenBank· DDBJ | AAM48425.1 EMBL· GenBank· DDBJ | mRNA |