B7YZV4 · PDE1_DROME

  • Protein
    Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1
  • Gene
    Pde1c
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Cyclic nucleotide phosphodiesterase with a dual specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes (PubMed:15673286).
Required for male fertility and male mating behavior (PubMed:20551439).

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions.
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 divalent metal cations per subunit. Site 2 has a preference for magnesium ions.

Activity regulation

Type I PDE are activated by the binding of calmodulin in the presence of Ca2+ (PubMed:15673286).
Inhibited by zaprinast and sildenafil (PubMed:15673286).

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
15.3 μM3',5'-cyclic GMP
20.5 μM3',5'-cyclic AMP

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site469Proton donor
Binding site473Zn2+ (UniProtKB | ChEBI)
Binding site509Zn2+ (UniProtKB | ChEBI)
Binding site510Mg2+ (UniProtKB | ChEBI)
Binding site510Zn2+ (UniProtKB | ChEBI)
Binding site617Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentextracellular space
Molecular Function3',5'-cGMP-inhibited cyclic-nucleotide phosphodiesterase activity
Molecular Function3',5'-cGMP-stimulated cyclic-nucleotide phosphodiesterase activity
Molecular Function3',5'-cyclic-AMP phosphodiesterase activity
Molecular Function3',5'-cyclic-GMP phosphodiesterase activity
Molecular Functioncalmodulin binding
Molecular Functioncalmodulin-activated 3',5'-cyclic-GMP phosphodiesterase activity
Molecular Functioncalmodulin-activated dual specificity 3',5'-cyclic-GMP, 3',5'-cyclic-AMP phosphodiesterase activity
Molecular Functionmetal ion binding
Biological ProcesscAMP metabolic process
Biological ProcesscAMP-mediated signaling
Biological ProcesscGMP metabolic process
Biological Processmale mating behavior
Biological Processsexual reproduction

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1
  • EC number
  • Short names
    PDE1

Gene names

    • Name
      Pde1c
    • ORF names
      CG44007

Organism names

  • Taxonomic identifier
  • Strain
    • Berkeley
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora

Accessions

  • Primary accession
    B7YZV4
  • Secondary accessions
    • Q9VKE9

Proteomes

Organism-specific databases

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004388611-834Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1

Proteomic databases

Expression

Tissue specificity

Expressed in the head (at protein level) (PubMed:15673286).
Expressed in Malpighian tubules (PubMed:15673286).
Expressed in neurons in the brain and ventral ganglia with male flies having higher levels of expression in the abdominal ganglia compared to female flies (PubMed:20551439).

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias, domain.

Type
IDPosition(s)Description
Region152-338Disordered
Compositional bias189-226Polar residues
Compositional bias258-300Polar residues
Region364-387Calmodulin-binding
Domain392-786PDEase
Region720-744Disordered
Region797-834Disordered

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

B7YZV4-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Name
    B
  • Synonyms
    G
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Length
    834
  • Mass (Da)
    91,455
  • Last updated
    2009-03-03 v1
  • Checksum
    D027A9481010C665
MQPSSPNATNYLADNIQISSANLSQTEMVVGRDSADYTAMHSINVGVGNSFLRGDTDIPQESGHSFETPSNMSFTAGQWDTESLPPVDTPDALNKAAGRIRSLLRRMDHETVAYEDMQRNLHYAARVLEAVFIDESREGCNGNCKNLNCSRHSHGRDDQQQDNNNSNRSCSLQEASPGGAGAGVTPGADNQDSIESRTKGVSQAPQTHSGPTGPPSNTSSETIAQPAPKLQPALETVRESVMEESPSKDPGDKGPPPPASTSTLTSQTTTSSSATAEPSAKAAESQAGSAGSSGSCSNPAAVHRQRRLRTPTWARSMSTNKTRLADEDDELSEVQPDAVPPEVREWLASTFTRQMATSRRKSDEKPKFRSVAHAIRAGIFVDRMYRRVSSSALTAFPPDVVRLLKNLDDWTFDVFALTEAASGQVVKYVAYELFNRYGSIHKFKIAPGILEAFLHRVEEGYCRYRNPYHNNLHAVDVMQTIHYCLCNTGLMNWLTDLEIFASLLAALLHDYEHTGTTNNFHVMSGSETALLYNDRAVLENHHASASFRLLREDEYNILSHLSREEFRELRGLVIEMVLGTDMTNHFQQMKAMRQLLTLQEATIDKQKVLSLVLHCCDISHPAKQWGVHHRWTMLLLEEFFRQGDLEKELGLPFSPLCDRNNTLVAESQICFIDFIVEPSMGVMSDMLELILAPIAPMNKSKPATLVEHETTANSTTNSAIVIPNSGITPSMDKPRDHRTEAKTTAAECLARKSVTGTTASKFNIPKPWLTCLVENKRIWKEQAVKDAEARALATAAEEAAAAAAAEAEESKPETETADGEQSEPAAEPADGAAA

B7YZV4-2

  • Name
    C
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-322: MQPSSPNATNYLADNIQISSANLSQTEMVVGRDSADYTAMHSINVGVGNSFLRGDTDIPQESGHSFETPSNMSFTAGQWDTESLPPVDTPDALNKAAGRIRSLLRRMDHETVAYEDMQRNLHYAARVLEAVFIDESREGCNGNCKNLNCSRHSHGRDDQQQDNNNSNRSCSLQEASPGGAGAGVTPGADNQDSIESRTKGVSQAPQTHSGPTGPPSNTSSETIAQPAPKLQPALETVRESVMEESPSKDPGDKGPPPPASTSTLTSQTTTSSSATAEPSAKAAESQAGSAGSSGSCSNPAAVHRQRRLRTPTWARSMSTNKT → MYEPGTSSEEGVVSETEPEPAGVSVSVSVSVSEEPSASRSSRLTVITVVVVVVAVRAMSRLRRESSRQSVITVELIQFIIQYRAKETQEKKKR

Computationally mapped potential isoform sequences

There are 6 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
B7YZV1B7YZV1_DROMEPde1c1137
B7YZV2B7YZV2_DROMEPde1c1173
B7YZV3B7YZV3_DROMEPde1c1009
M9PDA0M9PDA0_DROMEPde1c1158
M9NF02M9NF02_DROMEPde1c952
X2BZJ7X2BZJ7_DROMEPde1c855

Features

Showing features for alternative sequence, compositional bias.

Type
IDPosition(s)Description
Alternative sequenceVSP_0587521-322in isoform C
Compositional bias189-226Polar residues
Compositional bias258-300Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE014134
EMBL· GenBank· DDBJ
AAF53123.1
EMBL· GenBank· DDBJ
Genomic DNA
AE014134
EMBL· GenBank· DDBJ
ACL83022.1
EMBL· GenBank· DDBJ
Genomic DNA
AE014134
EMBL· GenBank· DDBJ
ACL83026.1
EMBL· GenBank· DDBJ
Genomic DNA
AY118396
EMBL· GenBank· DDBJ
AAM48425.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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