B7WS18 · B7WS18_COMTK
- ProteinATP-dependent 6-phosphofructokinase
- GenepfkA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids351 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Catalytic activity
- beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H+
Cofactor
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 10 | ATP (UniProtKB | ChEBI) | |||
Binding site | 105-108 | ATP (UniProtKB | ChEBI) | |||
Binding site | 106 | Mg2+ (UniProtKB | ChEBI); catalytic | |||
Site | 107 | Important for substrate specificity; cannot use PPi as phosphoryl donor | |||
Binding site | 128-130 | substrate; ligand shared between dimeric partners; in other chain | |||
Active site | 130 | Proton acceptor | |||
Binding site | 165 | substrate; ligand shared between dimeric partners | |||
Binding site | 172-174 | substrate; ligand shared between dimeric partners; in other chain | |||
Binding site | 224 | substrate; ligand shared between dimeric partners; in other chain | |||
Binding site | 268 | substrate; ligand shared between dimeric partners | |||
Binding site | 274-277 | substrate; ligand shared between dimeric partners; in other chain | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | 6-phosphofructokinase complex | |
Molecular Function | 6-phosphofructokinase activity | |
Molecular Function | AMP binding | |
Molecular Function | ATP binding | |
Molecular Function | diphosphate-fructose-6-phosphate 1-phosphotransferase activity | |
Molecular Function | fructose-6-phosphate binding | |
Molecular Function | identical protein binding | |
Molecular Function | metal ion binding | |
Molecular Function | monosaccharide binding | |
Biological Process | canonical glycolysis | |
Biological Process | fructose 1,6-bisphosphate metabolic process | |
Biological Process | fructose 6-phosphate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent 6-phosphofructokinase
- EC number
- Short namesATP-PFK ; Phosphofructokinase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Comamonadaceae > Comamonas
Accessions
- Primary accessionB7WS18
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer or homotetramer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 2-299 | Phosphofructokinase | |||
Sequence similarities
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length351
- Mass (Da)37,162
- Last updated2009-03-03 v1
- Checksum2055B26C6FCB5E9E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAUJ02000001 EMBL· GenBank· DDBJ | EED69072.1 EMBL· GenBank· DDBJ | Genomic DNA |