B7UX51 · HCHA_PSEA8
- ProteinProtein/nucleic acid deglycase HchA
- GenehchA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids291 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of Schiff bases and advanced glycation endproducts (AGE). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair. Plays an important role in protecting cells from carbonyl stress.
Catalytic activity
- H2O + N(omega)-(1-hydroxy-2-oxopropyl)-L-arginyl-[protein] = H+ + L-arginyl-[protein] + lactate
- H2O + N2-(1-hydroxy-2-oxopropyl)-dGTP = dGTP + H+ + lactate
- H2O + N2-(1-hydroxy-2-oxopropyl)-GTP = GTP + H+ + lactate
- H2O + N2-(1-hydroxy-2-oxopropyl)-GDP = GDP + H+ + lactate
- H2O + N2-(1-hydroxy-2-oxopropyl)-GMP = GMP + H+ + lactate
- H2O + N2-(1-hydroxy-2-oxoethyl)-dGTP = dGTP + glycolate + H+
- H2O + N2-(1-hydroxy-2-oxoethyl)-GTP = glycolate + GTP + H+
- H2O + N2-(1-hydroxy-2-oxoethyl)-GDP = GDP + glycolate + H+
- H2O + N2-(1-hydroxy-2-oxoethyl)-GMP = glycolate + GMP + H+
- an N2-(1-hydroxy-2-oxopropyl)-guanosine in RNA + H2O = a guanosine in RNA + H+ + lactate
- an N2-(1-hydroxy-2-oxopropyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + H+ + lactate
- an N2-(1-hydroxy-2-oxoethyl)-guanosine in RNA + H2O = a guanosine in RNA + glycolate + H+
- an N2-(1-hydroxy-2-oxoethyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + glycolate + H+
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 188 | Nucleophile | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | protein deglycase activity | |
Molecular Function | thiolester hydrolase activity | |
Biological Process | DNA repair | |
Biological Process | protein repair |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein/nucleic acid deglycase HchA
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionB7UX51
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000136182 | 1-291 | Protein/nucleic acid deglycase HchA | |||
Sequence: MSNERDTSRTPTPDHAEHNAFFPSPYSLSQYTSAKTDFDGADYPTPYKGGKKVLMIGTDERYILMQNGSMFSTGNHPVEMLLPMYHLDKAGFEFDVATLSGNPVKLEMWAMPGEDEAVKSIYAKYLPKLKAPQKLADLLEQAVADDSPYAAVFVPGGHGVLAGIPHSREVKRLLNAFLAKDRYIITLCHGPACLLAPAVEEKPEDYPFKDYEICVFPDALDTGANLEIGYMPGPLPWLVGENLQKLGVKILNKGITGQVHRDRKLLTGDSPLASNNLGKLAAKTLLEAFAR |
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length291
- Mass (Da)31,955
- Last updated2009-02-10 v1
- Checksum1F64F068E2B6035E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FM209186 EMBL· GenBank· DDBJ | CAW28939.1 EMBL· GenBank· DDBJ | Genomic DNA |