B7GRV8 · METE_BIFLS
- Protein5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
- GenemetE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids767 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.
Catalytic activity
- 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
Cofactor
Note: Binds 1 zinc ion per subunit.
Pathway
Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 17-20 | 5-methyltetrahydropteroyltri-L-glutamate (UniProtKB | ChEBI) | |||
Binding site | 117 | 5-methyltetrahydropteroyltri-L-glutamate (UniProtKB | ChEBI) | |||
Binding site | 441-443 | L-homocysteine (UniProtKB | ChEBI) | |||
Binding site | 441-443 | L-methionine (UniProtKB | ChEBI) | |||
Binding site | 494 | L-homocysteine (UniProtKB | ChEBI) | |||
Binding site | 494 | L-methionine (UniProtKB | ChEBI) | |||
Binding site | 525-526 | 5-methyltetrahydropteroyltri-L-glutamate (UniProtKB | ChEBI) | |||
Binding site | 571 | 5-methyltetrahydropteroyltri-L-glutamate (UniProtKB | ChEBI) | |||
Binding site | 609 | L-homocysteine (UniProtKB | ChEBI) | |||
Binding site | 609 | L-methionine (UniProtKB | ChEBI) | |||
Binding site | 615 | 5-methyltetrahydropteroyltri-L-glutamate (UniProtKB | ChEBI) | |||
Binding site | 652 | Zn2+ (UniProtKB | ChEBI); catalytic | |||
Binding site | 654 | Zn2+ (UniProtKB | ChEBI); catalytic | |||
Binding site | 676 | Zn2+ (UniProtKB | ChEBI); catalytic | |||
Active site | 705 | Proton donor | |||
Binding site | 737 | Zn2+ (UniProtKB | ChEBI); catalytic | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity | |
Molecular Function | zinc ion binding | |
Biological Process | methionine biosynthetic process | |
Biological Process | methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Bifidobacteriales > Bifidobacteriaceae > Bifidobacterium
Accessions
- Primary accessionB7GRV8
- Secondary accessions
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000123779 | 1-767 | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase | ||
Structure
Sequence
- Sequence statusComplete
- Length767
- Mass (Da)85,444
- Last updated2009-02-10 v1
- Checksum31ADD0BD14AD7192
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001095 EMBL· GenBank· DDBJ | ACJ52538.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP010889 EMBL· GenBank· DDBJ | BAJ69084.1 EMBL· GenBank· DDBJ | Genomic DNA |