B7GRV5 · GLNE_BIFLS
- ProteinBifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
- GeneglnE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1076 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.
Catalytic activity
- [glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP
Cofactor
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | [glutamate-ammonia-ligase] adenylyltransferase activity | |
Molecular Function | [glutamine synthetase]-adenylyl-L-tyrosine phosphorylase activity | |
Molecular Function | ATP binding | |
Molecular Function | magnesium ion binding | |
Biological Process | regulation of glutamine family amino acid metabolic process |
Keywords
- Molecular function
- Ligand
Names & Taxonomy
Protein names
- Recommended nameBifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
- Alternative names
Including 2 domains:
- Recommended nameGlutamine synthetase adenylyl-L-tyrosine phosphorylase
- EC number
- Alternative names
- Recommended nameGlutamine synthetase adenylyl transferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Bifidobacteriales > Bifidobacteriaceae > Bifidobacterium
Accessions
- Primary accessionB7GRV5
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000148541 | 1-1076 | Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme | ||
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-521 | Adenylyl removase | |||
Region | 524-1076 | Adenylyl transferase | |||
Region | 1041-1076 | Disordered | |||
Compositional bias | 1062-1076 | Basic and acidic residues | |||
Sequence similarities
Belongs to the GlnE family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,076
- Mass (Da)119,998
- Last updated2009-02-10 v1
- Checksum7283EB111BFC7999
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1062-1076 | Basic and acidic residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001095 EMBL· GenBank· DDBJ | ACJ52535.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP010889 EMBL· GenBank· DDBJ | BAJ69081.1 EMBL· GenBank· DDBJ | Genomic DNA |