B6ZB00 · B6ZB00_HPV58
- ProteinReplication protein E1
- GeneE1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids644 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 470-477 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell nucleus | |
Molecular Function | ATP binding | |
Molecular Function | DNA binding | |
Molecular Function | DNA helicase activity | |
Molecular Function | hydrolase activity, acting on acid anhydrides | |
Biological Process | DNA replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameReplication protein E1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageViruses > Monodnaviria > Shotokuvirae > Cossaviricota > Papovaviricetes > Zurhausenvirales > Papillomaviridae > Firstpapillomavirinae > Alphapapillomavirus > Alphapapillomavirus 9
- Virus hosts
Accessions
- Primary accessionB6ZB00
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for cross-link.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Cross-link | 551 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) | |||
Post-translational modification
Phosphorylated.
Sumoylated.
Keywords
- PTM
Expression
Keywords
- Developmental stage
Interaction
Subunit
Can form hexamers. Interacts with E2 protein; this interaction increases E1 DNA binding specificity. Interacts with host DNA polymerase subunit POLA2. Interacts with host single stranded DNA-binding protein RPA1. Interacts with host TOP1; this interaction stimulates the enzymatic activity of TOP1.
Family & Domains
Features
Showing features for motif, region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Motif | 86-88 | Nuclear localization signal | |||
Region | 146-177 | Disordered | |||
Region | 179-345 | DNA-binding region | |||
Domain | 444-594 | SF3 helicase | |||
Sequence similarities
Belongs to the papillomaviridae E1 protein family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length644
- Mass (Da)72,243
- Last updated2009-01-20 v1
- MD5 Checksum7B9184F4DFB1C85705E7117BDD0B9AF9
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FJ385261 EMBL· GenBank· DDBJ | ACJ13451.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
FJ385262 EMBL· GenBank· DDBJ | ACJ13459.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
FJ385265 EMBL· GenBank· DDBJ | ACJ13483.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
FJ385268 EMBL· GenBank· DDBJ | ACJ13507.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
HQ537757 EMBL· GenBank· DDBJ | AEI61648.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
HQ537759 EMBL· GenBank· DDBJ | AEI61664.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
HQ537760 EMBL· GenBank· DDBJ | AEI61672.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KC860270 EMBL· GenBank· DDBJ | AGQ21859.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY225918 EMBL· GenBank· DDBJ | ASQ41704.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY225919 EMBL· GenBank· DDBJ | ASQ41712.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY225936 EMBL· GenBank· DDBJ | ASQ41848.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY225937 EMBL· GenBank· DDBJ | ASQ41856.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY225939 EMBL· GenBank· DDBJ | ASQ41872.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY225941 EMBL· GenBank· DDBJ | ASQ41888.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY225944 EMBL· GenBank· DDBJ | ASQ41912.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY225945 EMBL· GenBank· DDBJ | ASQ41920.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY225946 EMBL· GenBank· DDBJ | ASQ41928.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY225947 EMBL· GenBank· DDBJ | ASQ41936.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY225948 EMBL· GenBank· DDBJ | ASQ41944.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY225950 EMBL· GenBank· DDBJ | ASQ41960.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY225951 EMBL· GenBank· DDBJ | ASQ41968.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY225952 EMBL· GenBank· DDBJ | ASQ41976.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY225953 EMBL· GenBank· DDBJ | ASQ41984.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY225954 EMBL· GenBank· DDBJ | ASQ41992.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY225955 EMBL· GenBank· DDBJ | ASQ42000.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB819279 EMBL· GenBank· DDBJ | BAN63332.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270077 EMBL· GenBank· DDBJ | BBA19984.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270078 EMBL· GenBank· DDBJ | BBA19992.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270081 EMBL· GenBank· DDBJ | BBA20016.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270083 EMBL· GenBank· DDBJ | BBA20032.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270084 EMBL· GenBank· DDBJ | BBA20040.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270085 EMBL· GenBank· DDBJ | BBA20048.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270088 EMBL· GenBank· DDBJ | BBA20072.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270091 EMBL· GenBank· DDBJ | BBA20096.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270096 EMBL· GenBank· DDBJ | BBA20136.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270098 EMBL· GenBank· DDBJ | BBA20152.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270099 EMBL· GenBank· DDBJ | BBA20160.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270101 EMBL· GenBank· DDBJ | BBA20176.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270103 EMBL· GenBank· DDBJ | BBA20192.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270106 EMBL· GenBank· DDBJ | BBA20216.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270110 EMBL· GenBank· DDBJ | BBA20248.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270113 EMBL· GenBank· DDBJ | BBA20272.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270115 EMBL· GenBank· DDBJ | BBA20288.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270117 EMBL· GenBank· DDBJ | BBA20304.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270121 EMBL· GenBank· DDBJ | BBA20336.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LC270123 EMBL· GenBank· DDBJ | BBA20352.1 EMBL· GenBank· DDBJ | Genomic DNA |