B6YXU4 · ENDA_THEON
- ProteintRNA-splicing endonuclease
- GeneendA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids171 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Catalytic activity
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 110 | |||||
Sequence: Y | ||||||
Active site | 117 | |||||
Sequence: H | ||||||
Active site | 148 | |||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | lyase activity | |
Molecular Function | nucleic acid binding | |
Molecular Function | tRNA-intron endonuclease activity | |
Biological Process | tRNA splicing, via endonucleolytic cleavage and ligation |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-splicing endonuclease
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Thermococci > Thermococcales > Thermococcaceae > Thermococcus
Accessions
- Primary accessionB6YXU4
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000188343 | 1-171 | tRNA-splicing endonuclease | |||
Sequence: MKEPIEFKLSGDRAFSEREKAINQLYNRRYFGEVVNGKLFLSLIEAAYLMERGKIKVLDGGKELSFEELFELGRKKDDQFDIKYLVYKDLRDRGYIVKSALKFGSHFRVYRRGMDEHSQWLIWVVPENLRFSANDITARVRVAHGVRKNMVMAVVDEDNDVVYYKIEWVKF |
Interaction
Subunit
Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length171
- Mass (Da)20,286
- Last updated2009-01-20 v1
- ChecksumBEFA08021C7A99D9
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000855 EMBL· GenBank· DDBJ | ACJ16907.1 EMBL· GenBank· DDBJ | Genomic DNA |