B6YXU4 · ENDA_THEON

Function

function

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.

Catalytic activity

  • pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.
    EC:4.6.1.16 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site.

117120406080100120140160
TypeIDPosition(s)Description
Active site110
Active site117
Active site148

GO annotations

AspectTerm
Molecular Functionlyase activity
Molecular Functionnucleic acid binding
Molecular FunctiontRNA-intron endonuclease activity
Biological ProcesstRNA splicing, via endonucleolytic cleavage and ligation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-splicing endonuclease
  • EC number
  • Alternative names
    • tRNA-intron endonuclease

Gene names

    • Name
      endA
    • Ordered locus names
      TON_1417

Organism names

Accessions

  • Primary accession
    B6YXU4

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10001883431-171tRNA-splicing endonuclease

Interaction

Subunit

Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    171
  • Mass (Da)
    20,286
  • Last updated
    2009-01-20 v1
  • Checksum
    BEFA08021C7A99D9
MKEPIEFKLSGDRAFSEREKAINQLYNRRYFGEVVNGKLFLSLIEAAYLMERGKIKVLDGGKELSFEELFELGRKKDDQFDIKYLVYKDLRDRGYIVKSALKFGSHFRVYRRGMDEHSQWLIWVVPENLRFSANDITARVRVAHGVRKNMVMAVVDEDNDVVYYKIEWVKF

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000855
EMBL· GenBank· DDBJ
ACJ16907.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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