B6VRE6 · A622L_TOBAC
- ProteinIsoflavone reductase homolog A622-like
- GeneA622L
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids310 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Involved in the biosynthesis of pyridine alkaloid natural products, leading mainly to the production of anabasine, anatabine, nicotine and nornicotine, effective deterrents against herbivores with antiparasitic and pesticide properties (neurotoxins); nornicotine serves as the precursor in the synthesis of the carcinogen compound N'-nitrosonornicotine (NNN) (PubMed:19002761).
Reductase that may be involved in a late step of tobacco alkaloid biosynthesis (PubMed:19002761).
Maybe involved in either the formation of a nicotinic acid-derived precursor or the final condensation reaction of tobacco alkaloids (PubMed:19002761).
Reductase that may be involved in a late step of tobacco alkaloid biosynthesis (PubMed:19002761).
Maybe involved in either the formation of a nicotinic acid-derived precursor or the final condensation reaction of tobacco alkaloids (PubMed:19002761).
Miscellaneous
Root cells silencing A622L exhibit inhibition of cell growth, severely decreased formation of several alkaloids, and accumulation of nicotinic acid beta-N-glucoside and N-methylpyrrolinium cation.
Pathway
Alkaloid biosynthesis; nicotine biosynthesis.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | oxidoreductase activity | |
Biological Process | alkaloid metabolic process | |
Biological Process | nicotine biosynthetic process | |
Biological Process | secondary metabolite biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameIsoflavone reductase homolog A622-like
- EC number
- Short namesNtA622L
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Nicotianoideae > Nicotianeae > Nicotiana
Accessions
- Primary accessionB6VRE6
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000442616 | 1-310 | Isoflavone reductase homolog A622-like | |||
Sequence: MVVSEKSKILIIGGTGYIGKYLVETSAKSGHPTFVLIRESTLVNPEKSKLIDTFKSYGVTLLFGDISNQESLLKAIKQVDVVISTVGGQQFADQVNIIKAIKEAGNIKRFLPSEFGFDVDHAHAIEPAASLFALKVKIRRMIEAEGIPYTYVICNWFADFFLPNLGQLEAKTPPRDKVVIFGDGNPKAIYVKEEDIATYTMKAVDDPRTLNKTLHMRPPANILSFNEIVSLWEEKIGKTLEKLYLSEEDILHIVQEGPMPLRVNLAICHSVFVNGDSANFEIQPSTGVEATELYPKVKYTTVDEYYNKFV |
Proteomic databases
Expression
Tissue specificity
Expressed in roots.
Induction
Induced by methyl jasmonate in roots.
Interaction
Structure
Sequence
- Sequence statusComplete
- Length310
- Mass (Da)34,652
- Last updated2009-01-20 v1
- ChecksumD2B8CC22168D9218
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB445396 EMBL· GenBank· DDBJ | BAG84265.1 EMBL· GenBank· DDBJ | mRNA | ||
AB445397 EMBL· GenBank· DDBJ | BAG84266.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KJ776597 EMBL· GenBank· DDBJ | AII71785.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KJ776598 EMBL· GenBank· DDBJ | AII71786.1 EMBL· GenBank· DDBJ | mRNA |