B6VAP9 · B6VAP9_PIG

  • Protein
    DNA repair nuclease/redox regulator APEX1
  • Gene
    APEX1
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    4/5

Function

function

Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.

Catalytic activity

  • Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.
    EC:3.1.11.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Probably binds two magnesium or manganese ions per subunit.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site, site.

131850100150200250300
TypeIDPosition(s)Description
Binding site68Mg2+ 1 (UniProtKB | ChEBI)
Binding site96Mg2+ 1 (UniProtKB | ChEBI)
Active site171
Active site210Proton donor/acceptor
Binding site210Mg2+ 2 (UniProtKB | ChEBI)
Binding site212Mg2+ 2 (UniProtKB | ChEBI)
Site212Transition state stabilizer
Site283Important for catalytic activity
Binding site308Mg2+ 1 (UniProtKB | ChEBI)
Active site309Proton acceptor
Binding site309Mg2+ 2 (UniProtKB | ChEBI)
Site309Interaction with DNA substrate

GO annotations

AspectTerm
Cellular Componentmitochondrion
Cellular Componentnucleus
Molecular FunctionDNA binding
Molecular Functionendonuclease activity
Molecular Functionexonuclease activity
Molecular Functionmetal ion binding
Biological ProcessDNA recombination
Biological ProcessDNA repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA repair nuclease/redox regulator APEX1
  • EC number
  • Short names
    APEN
    ; REF-1
  • Alternative names
    • APEX nuclease
    • Apurinic-apyrimidinic endonuclease 1
    • Redox factor-1
  • Cleaved into 1 chains

Gene names

    • Name
      APEX1

Organism names

  • Taxonomic identifier
  • Organism
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Suina > Suidae > Sus

Accessions

  • Primary accession
    B6VAP9

Subcellular Location

Nucleus
Cytoplasm
Mitochondrion

Keywords

Structure

Family & Domains

Features

Showing features for compositional bias, region, domain.

TypeIDPosition(s)Description
Compositional bias1-41Basic and acidic residues
Region1-59Disordered
Domain65-309Endonuclease/exonuclease/phosphatase

Sequence similarities

Belongs to the DNA repair enzymes AP/ExoA family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    318
  • Mass (Da)
    35,412
  • Last updated
    2008-12-16 v1
  • Checksum
    71B2AE0E95DFCA32
MPKRGKKAAVAEDGDEPKSEPEAKKSKAGAKKTEKEAAGEGAVLYEDPPDQKTSPSGKSATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSENKLPAELQELSGLPHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGEEEHDQEGRVIVAEFDAFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASHKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTAYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-41Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FJ358603
EMBL· GenBank· DDBJ
ACJ06403.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp