B6JMP4 · ISPH_HELP2
- Protein4-hydroxy-3-methylbut-2-enyl diphosphate reductase
- GeneispH
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids274 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.
Catalytic activity
- isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 reduced [2Fe-2S]-[ferredoxin] + 2 H+
Cofactor
Note: Binds 1 [4Fe-4S] cluster per subunit.
Pathway
Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 12 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 36 | (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 36 | dimethylallyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 36 | isopentenyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 70 | (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 70 | dimethylallyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 70 | isopentenyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 92 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 120 | (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 120 | dimethylallyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 120 | isopentenyl diphosphate (UniProtKB | ChEBI) | |||
Active site | 122 | Proton donor | |||
Binding site | 158 | (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 186 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 214 | (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 214 | dimethylallyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 214 | isopentenyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 215 | (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 215 | dimethylallyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 215 | isopentenyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 216 | (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 216 | dimethylallyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 216 | isopentenyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 258 | (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 258 | dimethylallyl diphosphate (UniProtKB | ChEBI) | |||
Binding site | 258 | isopentenyl diphosphate (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity | |
Molecular Function | metal ion binding | |
Biological Process | dimethylallyl diphosphate biosynthetic process | |
Biological Process | isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway | |
Biological Process | terpenoid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name4-hydroxy-3-methylbut-2-enyl diphosphate reductase
- EC number
- Short namesHMBPP reductase
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Campylobacterota > Epsilonproteobacteria > Campylobacterales > Helicobacteraceae > Helicobacter
Accessions
- Primary accessionB6JMP4
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000098953 | 1-274 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase | ||
Structure
Sequence
- Sequence statusComplete
- Length274
- Mass (Da)30,917
- Last updated2008-12-16 v1
- ChecksumFB08918117BA01BD
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001217 EMBL· GenBank· DDBJ | ACJ08172.1 EMBL· GenBank· DDBJ | Genomic DNA |