B6JMP4 · ISPH_HELP2

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site12[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site36(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site36dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site36isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site70(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site70dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site70isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site92[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site120(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site120dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site120isopentenyl diphosphate (UniProtKB | ChEBI)
Active site122Proton donor
Binding site158(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site186[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site214(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site214dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site214isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site215(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site215dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site215isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site216(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site216dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site216isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site258(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site258dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site258isopentenyl diphosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • Ordered locus names
      HPP12_1020

Organism names

Accessions

  • Primary accession
    B6JMP4

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10000989531-2744-hydroxy-3-methylbut-2-enyl diphosphate reductase

Structure

Family & Domains

Sequence similarities

Belongs to the IspH family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    274
  • Mass (Da)
    30,917
  • Last updated
    2008-12-16 v1
  • Checksum
    FB08918117BA01BD
MEIKMAKDYGFCFGVKRAIQIAEKNQNSLIFGSLIHNAKEINRLEKNFNVKIEEDPKKIPKNKSVIIRTHGIPKQDLEYLKNKGVKITDATCPYVIKPQQIVESMSKEGYQIVLFGDINHPEVKGVISYATNQALVVNSLEELQEKKLQRKVALVSQTTKQTPKLLQIASYLVERCTEVRIFNTICNATSYNQKAALDLSKEVDIMIVVGGKTSSNTKQLLSIAKQHCKDSYLVEDENELELAWFKDKKLCGITAGASTPDWIIENVKQKISTI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001217
EMBL· GenBank· DDBJ
ACJ08172.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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