B6JMA1 · RUVC_HELP2
- ProteinCrossover junction endodeoxyribonuclease RuvC
- GeneruvC
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids157 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair. Endonuclease that resolves HJ intermediates. Cleaves cruciform DNA by making single-stranded nicks across the HJ at symmetrical positions within the homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction. The consensus cleavage sequence is 5'-(A/T)TT(C/G)-3'. Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange. HJ branch migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.
Catalytic activity
Cofactor
Note: Binds 2 Mg2+ ion per subunit.
Features
Showing features for active site, binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | Holliday junction resolvase complex | |
Molecular Function | crossover junction DNA endonuclease activity | |
Molecular Function | DNA binding | |
Molecular Function | magnesium ion binding | |
Biological Process | DNA recombination | |
Biological Process | DNA repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCrossover junction endodeoxyribonuclease RuvC
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Campylobacterota > Epsilonproteobacteria > Campylobacterales > Helicobacteraceae > Helicobacter
Accessions
- Primary accessionB6JMA1
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000090532 | 1-157 | Crossover junction endodeoxyribonuclease RuvC | |||
Sequence: MRILGIDPGSRKCGYAIISHTSNKLSLITAGFINITTTRLQEQILDLIEALDCLLDRYEVHEVAIEDIFFAYNPKSVIKLAQFRGALSLKILERIGNFSEYTPLQVKKALTGNGKAAKEQVAFMVKRLLNITSEIKPLDISDAIAVAITHAQRLKLH |
Interaction
Subunit
Homodimer which binds Holliday junction (HJ) DNA. The HJ becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it has a different conformation from HJ DNA in complex with RuvA. In the full resolvosome a probable DNA-RuvA4-RuvB12-RuvC2 complex forms which resolves the HJ.
Structure
Sequence
- Sequence statusComplete
- Length157
- Mass (Da)17,492
- Last updated2008-12-16 v1
- Checksum0166F08CEAAC4E52
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001217 EMBL· GenBank· DDBJ | ACJ08029.1 EMBL· GenBank· DDBJ | Genomic DNA |