B6HLP9 · CHYA_PENRW

Function

function

Nonribosomal peptide synthase; part of the gene cluster that mediates the biosynthesis of the yellow pigment chrysogine (PubMed:29196288).
the NRPS chyA mediates the condensation of anthranilic acid and alanine into the intermediate 2-(2-aminopropanamido)benzoic acid (PubMed:29196288).
The remainder of the pathway is highly branched yielding at least 13 chrysogine-related compounds (PubMed:29196288).
The malonyl transferase chyE converts 2-(2-aminopropanamido)benzoic acid and 2-(2-aminopropanamido)benzamidine into 2-(2-(2-carboxyacetamido)propanamido)benzoic acid and 3-((1-((2-carbamoylphenyl)amino)-1-oxopropan-2-yl)amino)-3-oxopropanoic acid, respectively (PubMed:29196288).
ChyD is an amidase, being responsible for the amidation of the carboxylic acid moiety of 2-(2-aminopropanamido)benzoic acid, 2-(2-(2-carboxyacetamido)propanamido)benzoic acid and 2-(2-((4-amino-1-carboxy-4-oxobutyl)amino)propanamido)benzoic acid (PubMed:29196288).
ChyC is involved in the same reactions as ChyD, but plays a more minor role in the amidation reactions compared to chyD (PubMed:29196288).
The oxidoreductases chyH and chyM are involved in oxidation reactions that form N-pyruvoylanthranilamide from 2-(2-aminopropanamido)benzamidine and (1-((2-carbamoylphenyl)amino)-1-oxopropan-2-yl)glutamine, respectively (PubMed:29196288).
N-pyruvoylanthranilamide is further converted via two further branches in the pathway, yielding chrysogine and additional chrysogine-related coumpounds (PubMed:29196288).
Chrysogine is likely formed by a spontaneous ring closure from N-pyruvoylanthranilamide (PubMed:29196288).

Pathway

Pigment biosynthesis.

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionligase activity
Molecular Functionphosphopantetheine binding
Molecular Functiontransferase activity
Biological Processamino acid activation for nonribosomal peptide biosynthetic process
Biological Processsecondary metabolite biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Nonribosomal peptide synthase chyA
  • EC number
  • Short names
    NRPS chyA
  • Alternative names
    • Chrysogine biosynthesis cluster protein A

Gene names

    • Name
      chyA
    • ORF names
      Pc21g12630

Organism names

Accessions

  • Primary accession
    B6HLP9

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Impairs the production of chrysogine and 13 other chrysogine-related compounds (PubMed:29196288).

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00004433451-2382Nonribosomal peptide synthase chyA
Modified residue782O-(pantetheine 4'-phosphoryl)serine
Modified residue1870O-(pantetheine 4'-phosphoryl)serine

Keywords

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region204-607Adenylation 1
Domain745-821Carrier 1
Region857-1269Condensation 1
Region1294-1687Adenylation 2
Domain1833-1909Carrier 2
Region1967-2373Condensation 2

Domain

NRP synthetases are composed of discrete domains (adenylation (A), thiolation (T) or peptidyl carrier protein (PCP) and condensation (C) domains) which when grouped together are referred to as a single module. Each module is responsible for the recognition (via the A domain) and incorporation of a single amino acid into the growing peptide product. Thus, an NRP synthetase is generally composed of one or more modules and can terminate in a thioesterase domain (TE) that releases the newly synthesized peptide from the enzyme. Occasionally, epimerase (E) domains (responsible for L- to D-amino acid conversion) are present within the NRP synthetase. ChyA has the following architecture: A-T-C-A-T-C.

Sequence similarities

Belongs to the NRP synthetase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,382
  • Mass (Da)
    260,769
  • Last updated
    2008-12-16 v1
  • Checksum
    582DAC279F7F88E7
MAAPSISPLFAPQMGVQRDFQDSEMQIATVNFPCTGQSNTSLANWLQQEEKSHLQLLQAAWSISLRSYTGSNDVLFSCLNTKDTAALGANSVVYVDEGNEDKYGSARRGNLREFDLAICFSKQNEANCPVIGIHHKPSVISTDFATMIAATVAKAIEEIVLHVDSLIASLDICSDADINCISRWNSPSDDGIPSAQCIHHIISQKCATQPESIAVSAWDGRLTYAELDGLSSSLAIRLQHLGVCQEIFVPLIFDKSKWAVIALLSVLKAGGAYFFLNPSNPIQYNLGLCSSLSPEVALCSPRHSTLAKSFAGTAIPVGEEHCELPESLPVDEKTPPCTAETTPSNAMYITFTSGTTGVPKGITTEHSAFYSMAMANGKALQVGPATRMLQFASYTFDVSNRDMLITLMFGGCICIPSELDRLNDLSGFINRQSVNLASLTPSLASTLNPALCPSLQGLVLGGESMNDSHISAWANHVRLFNAYGVSESAGIAALASDIQADYSPGNIGFGSGSTLWVVTIDQPDKLAPIGALGEMVIEGPSVARGYLGDKKRTEEQFTSTSKWKNRIRAQLSESRSSKRAFHTGDLVRYNLDGSLNFLGRKDHQVKIHGQRLELTAIEHHIAACLEVAESGFLHVAVVTAKNEGNGSVKLLAFLGLYTSRGSDSPSQLVSKKLEDVEALKVALRQHLLLCLPAFMVPVDFIFVQHMPLTTSGKINRLLLQEAAGHALLDDQKRNIDTSDLNGNQTPTTQNQRILIQSWAKALGIKSESIMRNDCFFRRGGDSIAAIKMAASLRQQELIISVSDVFKFSTFSDMASVLVKDHRPLQTAMLAPFSLIDNSQTVLDAVMEELGTGIDQIEDVYPCTHMQQGLIALTAQQPHSYIGRYTWQLAEMLDVEKFKNAWESAWLHNPILRTRIVQIPDGVFQVVVKTDMPWNTVTDISRGGDGKELREIDISNGPLIQFYLSKESFRLDIHHSLFDEWSLGLIMGQVERAYAGGGLRMQPFSPFVQHLLHERDTSLEDFWRQEFSGLQAEHFPAIASRPLSVEHPTEKVVLEHSVQLETGFSTKYTISSIVRLAWAIVLWHQTGSEDVVFGATVSGRNANIDGIDQLSGPTLATLPVRIKLAASQPIHEGLSQVQGQFVNMMVHEQAGLPRIRQVGREAAEACNFQNLLVVQPYEEQTESHMFKASANSASSSENAKSFASYPMVLICRPEKSGISMKAAFDPAILTPAAGHSILKQMSHVIQQLVTSDSTCIAAVSLVPPEDMATLRQWNHSLPNGVNTCIHARIQELCIGQPDTLAIHSQNLDLTYGQLDNYSDQFAQNLIGSGVKQGDFVPLFLERSPWVPVIMLAVLKTGAAFVLLDLSHPMQRLRTMCSMIDARIVVTSKEHADRSGNLLLPVIIFDPEAHAQNVSKQATAPELKPLTAVTTPDAPACVVFSSGSTGLPKGIVLPHSALTTSAAVMREYGMLGPKSRVFHFASFAFDISIGEILFTLAAGACVCVPHEEERKGNPAKAAGDLKVTWALLTPSVINLFDPSDVPTLEVLGSAGEPLTPQIVDTWAHRVKLYGMYAPAECTVISHIGRILPDTHHSNIGKSHGGVSWVVDPSDHNRLVPIGTVGELIVEGPTVSSGYLNDPAKTNEVFITSPSWLDEVRSHSGKMYKTGDLVRQTSEGSLEFVGRKDDQVKLHGQRLEVGEVEHCITSSCTAIKTATVECIKIREQNSRVSLVAFICPQTDEDWGQSLNDPSSEVGDLELISPPRDQFYSMIESLETSLRELLPAYMVPSFFVPLADVPLSLSGKVNRRLLRDQSTSWPMKRLGLYQLRRKSIPAEEVPVSIHGRKVQEIVGQALNLDPKSIPMNSNFFGLGGDSISAMQVSMLARRRGIRLTVADIFTQQTLSGLSLKCATENGDASQASKSRSLGRELPGSNIKSLHRCEIPRDKLPRQLPQEIADNIVEAMPATEFQTMTLHNFYSRYLWISLPERVNQEHLLNACDQLVQKHSVLRTVFYTNDDKSVVQLTLRKVPVNFVHYSNIENLEKHCADDSLAMGVPINSVPGFEVQLVTLRDSGMYLILRLPHAQFDGVSLDIICSDLSAAYSGDSLPPCAQFSDHIRHVWEKRIPESYNAWREVLGNVPMTSLNNKYLRNWGSASEMGSPNMGTDPDQPKVVTAMAETLPISPPPNITLATLVKLAWAITLSRLFTSIEEDDGASDDVVFGQVVHGRGLGISHEDRIVGPCLNIIPVRVHLPPRSNKLDLLGQVQQQHIQTMSVENLELGEITRNCTSWKAGTKFGSFIRFQNFTNNDDSTCSFDGSACETGLYSLPNRPSNTANVLVVPHGPTLSITMTISNQVLDRGSADFVAGYFSDVIESLASEETVCEYLE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AM920436
EMBL· GenBank· DDBJ
CAP96160.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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