B6HLP6 · CHYC_PENRW

Function

function

Short-chain dehydrogenase; part of the gene cluster that mediates the biosynthesis of the yellow pigment chrysogine (PubMed:29196288).
the NRPS chyA mediates the condensation of anthranilic acid and alanine into the intermediate 2-(2-aminopropanamido)benzoic acid (PubMed:29196288).
The remainder of the pathway is highly branched yielding at least 13 chrysogine-related compounds (PubMed:29196288).
The malonyl transferase chyE converts 2-(2-aminopropanamido)benzoic acid and 2-(2-aminopropanamido)benzamidine into 2-(2-(2-carboxyacetamido)propanamido)benzoic acid and 3-((1-((2-carbamoylphenyl)amino)-1-oxopropan-2-yl)amino)-3-oxopropanoic acid, respectively (PubMed:29196288).
ChyD is an amidase, being responsible for the amidation of the carboxylic acid moiety of 2-(2-aminopropanamido)benzoic acid, 2-(2-(2-carboxyacetamido)propanamido)benzoic acid and 2-(2-((4-amino-1-carboxy-4-oxobutyl)amino)propanamido)benzoic acid (PubMed:29196288).
ChyC is involved in the same reactions as ChyD, but plays a more minor role in the amidation reactions compared to chyD (PubMed:29196288).
The oxidoreductases chyH and chyM are involved in oxidation reactions that form N-pyruvoylanthranilamide from 2-(2-aminopropanamido)benzamidine and (1-((2-carbamoylphenyl)amino)-1-oxopropan-2-yl)glutamine, respectively (PubMed:29196288).
N-pyruvoylanthranilamide is further converted via two further branches in the pathway, yielding chrysogine and additional chrysogine-related coumpounds (PubMed:29196288).
Chrysogine is likely formed by a spontaneous ring closure from N-pyruvoylanthranilamide (PubMed:29196288).

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site37NADP+ (UniProtKB | ChEBI)
Binding site55NADP+ (UniProtKB | ChEBI)
Binding site81NADP+ (UniProtKB | ChEBI)
Active site154Proton donor
Binding site154NADP+ (UniProtKB | ChEBI)
Active site158Lowers pKa of active site Tyr
Binding site158NADP+ (UniProtKB | ChEBI)
Binding site185NADP+ (UniProtKB | ChEBI)
Binding site187NADP+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionoxidoreductase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Short-chain dehydrogenase chyC
  • EC number
  • Alternative names
    • Chrysogine biosynthesis cluster protein C

Gene names

    • Name
      chyC
    • ORF names
      Pc21g12600

Organism names

Accessions

  • Primary accession
    B6HLP6

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Accumulates 2-(2-aminopropanamido)benzoic acid, 2-(2-(2-carboxyacetamido)propanamido)benzoic acid and 2-(2-((4-amino-1-carboxy-4-oxobutyl)amino)propanamido)benzoic acid, but downstream compounds are still produced in low amount (PubMed:29196288).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004433461-258Short-chain dehydrogenase chyC

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    258
  • Mass (Da)
    28,245
  • Last updated
    2008-12-16 v1
  • MD5 Checksum
    BFAEFD1F2145077EDB98C78B6A521D8B
MARILITGSTDGFGLEAARQLVDRKHVVYLHARSQERAEEVKTKCPGAAGVLVADLTSVAETRKLAEEANAIGTFDAIILNAGLLYGPFRKTDYGVPAMPFVNVLAPYILSCLLEQPKRLIFIASILHKEAKTDLKDIFWLERGEKEFQDFPAYCDSKFHVMLLANAVAKRFKGTSVTSVHPGYVATKLGGSGATDKMEDGVETYVMLAEGDYDQSLTGVYFVPKKQIGEPLPETTQEDLQETVVKACEDITGIKLPA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AM920436
EMBL· GenBank· DDBJ
CAP96157.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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