B6HLP6 · CHYC_PENRW
- ProteinShort-chain dehydrogenase chyC
- GenechyC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids258 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Short-chain dehydrogenase; part of the gene cluster that mediates the biosynthesis of the yellow pigment chrysogine (PubMed:29196288).
the NRPS chyA mediates the condensation of anthranilic acid and alanine into the intermediate 2-(2-aminopropanamido)benzoic acid (PubMed:29196288).
The remainder of the pathway is highly branched yielding at least 13 chrysogine-related compounds (PubMed:29196288).
The malonyl transferase chyE converts 2-(2-aminopropanamido)benzoic acid and 2-(2-aminopropanamido)benzamidine into 2-(2-(2-carboxyacetamido)propanamido)benzoic acid and 3-((1-((2-carbamoylphenyl)amino)-1-oxopropan-2-yl)amino)-3-oxopropanoic acid, respectively (PubMed:29196288).
ChyD is an amidase, being responsible for the amidation of the carboxylic acid moiety of 2-(2-aminopropanamido)benzoic acid, 2-(2-(2-carboxyacetamido)propanamido)benzoic acid and 2-(2-((4-amino-1-carboxy-4-oxobutyl)amino)propanamido)benzoic acid (PubMed:29196288).
ChyC is involved in the same reactions as ChyD, but plays a more minor role in the amidation reactions compared to chyD (PubMed:29196288).
The oxidoreductases chyH and chyM are involved in oxidation reactions that form N-pyruvoylanthranilamide from 2-(2-aminopropanamido)benzamidine and (1-((2-carbamoylphenyl)amino)-1-oxopropan-2-yl)glutamine, respectively (PubMed:29196288).
N-pyruvoylanthranilamide is further converted via two further branches in the pathway, yielding chrysogine and additional chrysogine-related coumpounds (PubMed:29196288).
Chrysogine is likely formed by a spontaneous ring closure from N-pyruvoylanthranilamide (PubMed:29196288).
the NRPS chyA mediates the condensation of anthranilic acid and alanine into the intermediate 2-(2-aminopropanamido)benzoic acid (PubMed:29196288).
The remainder of the pathway is highly branched yielding at least 13 chrysogine-related compounds (PubMed:29196288).
The malonyl transferase chyE converts 2-(2-aminopropanamido)benzoic acid and 2-(2-aminopropanamido)benzamidine into 2-(2-(2-carboxyacetamido)propanamido)benzoic acid and 3-((1-((2-carbamoylphenyl)amino)-1-oxopropan-2-yl)amino)-3-oxopropanoic acid, respectively (PubMed:29196288).
ChyD is an amidase, being responsible for the amidation of the carboxylic acid moiety of 2-(2-aminopropanamido)benzoic acid, 2-(2-(2-carboxyacetamido)propanamido)benzoic acid and 2-(2-((4-amino-1-carboxy-4-oxobutyl)amino)propanamido)benzoic acid (PubMed:29196288).
ChyC is involved in the same reactions as ChyD, but plays a more minor role in the amidation reactions compared to chyD (PubMed:29196288).
The oxidoreductases chyH and chyM are involved in oxidation reactions that form N-pyruvoylanthranilamide from 2-(2-aminopropanamido)benzamidine and (1-((2-carbamoylphenyl)amino)-1-oxopropan-2-yl)glutamine, respectively (PubMed:29196288).
N-pyruvoylanthranilamide is further converted via two further branches in the pathway, yielding chrysogine and additional chrysogine-related coumpounds (PubMed:29196288).
Chrysogine is likely formed by a spontaneous ring closure from N-pyruvoylanthranilamide (PubMed:29196288).
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 37 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 55 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 81 | NADP+ (UniProtKB | ChEBI) | |||
Active site | 154 | Proton donor | |||
Binding site | 154 | NADP+ (UniProtKB | ChEBI) | |||
Active site | 158 | Lowers pKa of active site Tyr | |||
Binding site | 158 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 185 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 187 | NADP+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | oxidoreductase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameShort-chain dehydrogenase chyC
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Penicillium > Penicillium chrysogenum species complex
Accessions
- Primary accessionB6HLP6
Proteomes
Organism-specific databases
Phenotypes & Variants
Disruption phenotype
Accumulates 2-(2-aminopropanamido)benzoic acid, 2-(2-(2-carboxyacetamido)propanamido)benzoic acid and 2-(2-((4-amino-1-carboxy-4-oxobutyl)amino)propanamido)benzoic acid, but downstream compounds are still produced in low amount (PubMed:29196288).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000443346 | 1-258 | Short-chain dehydrogenase chyC | ||
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length258
- Mass (Da)28,245
- Last updated2008-12-16 v1
- MD5 ChecksumBFAEFD1F2145077EDB98C78B6A521D8B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AM920436 EMBL· GenBank· DDBJ | CAP96157.1 EMBL· GenBank· DDBJ | Genomic DNA |