B5Z1D1 · NRFA_ECO5E

Function

function

Catalyzes the reduction of nitrite to ammonia, consuming six electrons in the process.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Ca2+ (UniProtKB | Rhea| CHEBI:29108 )

Note: Binds 1 Ca2+ ion per monomer.
heme c (UniProtKB | Rhea| CHEBI:61717 )

Note: Binds 5 heme c groups covalently per monomer.

Pathway

Nitrogen metabolism; nitrate reduction (assimilation).

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site94Fe (UniProtKB | ChEBI) of heme c 3 (UniProtKB | ChEBI); axial binding residue
Binding site122heme 1 (UniProtKB | ChEBI); covalent
Binding site125heme 1 (UniProtKB | ChEBI); covalent
Binding site126Fe (UniProtKB | ChEBI) of heme 1 (UniProtKB | ChEBI); axial binding residue
Binding site160heme c 2 (UniProtKB | ChEBI); covalent
Binding site163heme c 2 (UniProtKB | ChEBI); covalent
Binding site164Fe (UniProtKB | ChEBI) of heme c 2 (UniProtKB | ChEBI); axial binding residue
Binding site209heme c 3 (UniProtKB | ChEBI); covalent
Binding site212heme c 3 (UniProtKB | ChEBI); covalent
Binding site213Fe (UniProtKB | ChEBI) of heme c 3 (UniProtKB | ChEBI); axial binding residue
Binding site215Ca2+ (UniProtKB | ChEBI)
Binding site216Ca2+ (UniProtKB | ChEBI)
Binding site216substrate
Binding site261Ca2+ (UniProtKB | ChEBI)
Binding site263Ca2+ (UniProtKB | ChEBI)
Binding site264substrate
Binding site275Fe (UniProtKB | ChEBI) of heme c 5 (UniProtKB | ChEBI); axial binding residue
Binding site282heme c 4 (UniProtKB | ChEBI); covalent
Binding site285heme c 4 (UniProtKB | ChEBI); covalent
Binding site286Fe (UniProtKB | ChEBI) of heme c 4 (UniProtKB | ChEBI); axial binding residue
Binding site301Fe (UniProtKB | ChEBI) of heme c 2 (UniProtKB | ChEBI); axial binding residue
Binding site314heme c 5 (UniProtKB | ChEBI); covalent
Binding site317heme c 5 (UniProtKB | ChEBI); covalent
Binding site318Fe (UniProtKB | ChEBI) of heme c 5 (UniProtKB | ChEBI); axial binding residue
Binding site393Fe (UniProtKB | ChEBI) of heme c 4 (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentouter membrane-bounded periplasmic space
Molecular Functioncalcium ion binding
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionnitrite reductase (cytochrome, ammonia-forming) activity
Biological Processanaerobic electron transport chain
Biological Processnitrate assimilation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytochrome c-552
  • EC number
  • Alternative names
    • Ammonia-forming cytochrome c nitrite reductase
      (Cytochrome c nitrite reductase
      )

Gene names

    • Name
      nrfA
    • Ordered locus names
      ECH74115_5575

Organism names

Accessions

  • Primary accession
    B5Z1D1

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-26
ChainPRO_100013821027-478Cytochrome c-552

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome c-552 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    478
  • Mass (Da)
    53,684
  • Last updated
    2008-11-25 v1
  • Checksum
    5BB3744E2679B52D
MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNETFAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSHDYNKPRGHAFAVTDVRETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGCADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYFDGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHGKNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQSINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHMHAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQIKAEKQDFIKTVIPQWEEQARKNGLLSQ

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001164
EMBL· GenBank· DDBJ
ACI38126.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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