B5VJA0 · CHO2_YEAS6
- ProteinPhosphatidylethanolamine N-methyltransferase
- GeneCHO2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids869 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the first step of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylethanolamine (PE) to phosphatidylmonomethylethanolamine (PMME).
Catalytic activity
- a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + S-adenosyl-L-methionine = 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine + H+ + S-adenosyl-L-homocysteine
Pathway
Phospholipid metabolism; phosphatidylcholine biosynthesis.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | phosphatidylethanolamine N-methyltransferase activity | |
Biological Process | methylation | |
Biological Process | phosphatidylcholine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphatidylethanolamine N-methyltransferase
- EC number
- Short namesPE methyltransferase ; PEAMT ; PEMT
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionB5VJA0
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 2-55 | Lumenal | ||||
Sequence: SSCKTTLSEMVGSVTKDRGTINVKARTRSSNVTFKPPVTHDMVRSLFDPTLKKS | ||||||
Transmembrane | 56-76 | Helical | ||||
Sequence: LLEKCIALAIISNFFICYWVF | ||||||
Topological domain | 77-86 | Cytoplasmic | ||||
Sequence: QRFGLQFTKY | ||||||
Transmembrane | 87-107 | Helical | ||||
Sequence: FFLVQYLFWRIAYNLGIGLVL | ||||||
Topological domain | 108-187 | Lumenal | ||||
Sequence: HYQSHYETLTNCAKTHAIFSKIPHNKDANSNFSTNSNSFSEKFWNFIRKFCQYEIRSKMPKEYDLFAYPEEINVWLIFRQ | ||||||
Transmembrane | 188-208 | Helical | ||||
Sequence: FVDLILMQDFVTYIIYVYLSI | ||||||
Topological domain | 209-212 | Cytoplasmic | ||||
Sequence: PYSW | ||||||
Transmembrane | 213-233 | Helical | ||||
Sequence: VQIFNWRSLLGVILILFNIWV | ||||||
Topological domain | 234-258 | Lumenal | ||||
Sequence: KLDAHRVVKDYAWYWGDFFFLEESE | ||||||
Transmembrane | 259-279 | Helical | ||||
Sequence: LIFDGVFNISPHPMYSIGYLG | ||||||
Topological domain | 280-291 | Cytoplasmic | ||||
Sequence: YYGLSLICNDYK | ||||||
Transmembrane | 292-310 | Helical | ||||
Sequence: VLLVSVFGHYSQFLFLKYV | ||||||
Topological domain | 311-362 | Lumenal | ||||
Sequence: ENPHIERTYGDGTDSDSQMNSRIDDLISKENYDYSRPLINMGLSFNNFNKLR | ||||||
Transmembrane | 363-383 | Helical | ||||
Sequence: FTDYFTIGTVAALMLGTIMNA | ||||||
Topological domain | 384-389 | Cytoplasmic | ||||
Sequence: RFINLN | ||||||
Transmembrane | 390-410 | Helical | ||||
Sequence: YLFITVFVTKLVSWLFISTIL | ||||||
Topological domain | 411-439 | Lumenal | ||||
Sequence: YKQSQSKWFTRLFLENGYTQVYSYEQWQF | ||||||
Transmembrane | 440-460 | Helical | ||||
Sequence: IYNYYLVLTYTLMIIYTGLQI | ||||||
Topological domain | 461-463 | Cytoplasmic | ||||
Sequence: WSN | ||||||
Transmembrane | 464-484 | Helical | ||||
Sequence: FSNINNSQLIFGLILVALQTW | ||||||
Topological domain | 485-534 | Lumenal | ||||
Sequence: CDKETRLAISDFGWFYGDFFLSNYISTRKLTSQGIYRYLNHPEAVLGVVG | ||||||
Transmembrane | 535-555 | Helical | ||||
Sequence: VWGTVLMTNFAVTNIILAVLW | ||||||
Topological domain | 556-869 | Cytoplasmic | ||||
Sequence: TLTNFILVKFIETPHVNKIYGKTKRVSGVGKTLLGLKPLRQVSDIVNRIENIIIKSLVDESKNSNGGAELLPKNYQDNKEWNILIQEAMDSVATRLSPYCELKIENEQIETNFVLPTPVTLNWKMPIELYNGDDWIGLYKVIDTRADREKTRVGSGGHWSATSKDSYMNHGLRHKESVTEIKATEKYVQGKVTFDTSLLYFENGIYEFRYHSGNSHKVLLISTPFEISLPVLNTTTPELFEKDLTEFLTKVNVLKDGKFRPLGNKFFGMDSLKQLIKNSIGVELSSEYMRRVNGDAHVISHRAWDIKQTLDSLA |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylserine | ||||
Sequence: S | ||||||
Chain | PRO_0000405912 | 2-869 | Phosphatidylethanolamine N-methyltransferase | |||
Sequence: SSCKTTLSEMVGSVTKDRGTINVKARTRSSNVTFKPPVTHDMVRSLFDPTLKKSLLEKCIALAIISNFFICYWVFQRFGLQFTKYFFLVQYLFWRIAYNLGIGLVLHYQSHYETLTNCAKTHAIFSKIPHNKDANSNFSTNSNSFSEKFWNFIRKFCQYEIRSKMPKEYDLFAYPEEINVWLIFRQFVDLILMQDFVTYIIYVYLSIPYSWVQIFNWRSLLGVILILFNIWVKLDAHRVVKDYAWYWGDFFFLEESELIFDGVFNISPHPMYSIGYLGYYGLSLICNDYKVLLVSVFGHYSQFLFLKYVENPHIERTYGDGTDSDSQMNSRIDDLISKENYDYSRPLINMGLSFNNFNKLRFTDYFTIGTVAALMLGTIMNARFINLNYLFITVFVTKLVSWLFISTILYKQSQSKWFTRLFLENGYTQVYSYEQWQFIYNYYLVLTYTLMIIYTGLQIWSNFSNINNSQLIFGLILVALQTWCDKETRLAISDFGWFYGDFFLSNYISTRKLTSQGIYRYLNHPEAVLGVVGVWGTVLMTNFAVTNIILAVLWTLTNFILVKFIETPHVNKIYGKTKRVSGVGKTLLGLKPLRQVSDIVNRIENIIIKSLVDESKNSNGGAELLPKNYQDNKEWNILIQEAMDSVATRLSPYCELKIENEQIETNFVLPTPVTLNWKMPIELYNGDDWIGLYKVIDTRADREKTRVGSGGHWSATSKDSYMNHGLRHKESVTEIKATEKYVQGKVTFDTSLLYFENGIYEFRYHSGNSHKVLLISTPFEISLPVLNTTTPELFEKDLTEFLTKVNVLKDGKFRPLGNKFFGMDSLKQLIKNSIGVELSSEYMRRVNGDAHVISHRAWDIKQTLDSLA |
Keywords
- PTM
Structure
Family & Domains
Sequence similarities
Belongs to the class VI-like SAM-binding methyltransferase superfamily. CHO2 family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length869
- Mass (Da)101,252
- Last updated2008-11-25 v1
- ChecksumA6598AB6B817C112