B4R8T7 · ISPH_PHEZH

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site18[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site47(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site47dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site47isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site80(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site80dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site80isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site102[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site130(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site130dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site130isopentenyl diphosphate (UniProtKB | ChEBI)
Active site132Proton donor
Binding site171(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site201[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site229(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site229dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site229isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site230(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site230dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site230isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site231(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site231dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site231isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site273(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site273dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site273isopentenyl diphosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • Ordered locus names
      PHZ_c2893

Organism names

Accessions

  • Primary accession
    B4R8T7

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10000989621-3144-hydroxy-3-methylbut-2-enyl diphosphate reductase

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the IspH family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    314
  • Mass (Da)
    33,628
  • Last updated
    2008-09-23 v1
  • Checksum
    E35291BB8E54ED42
MPSRPPLTVLLASPRGFCAGVDRAIQIVERAIEKYGAPVYVRHEIVHNRHVVERLKALGAVFVEELDQAPDDRPVVFSAHGVPKSVPAAAKSRRMLYLDATCPLVSKVHVEAQRHFDAGREIVLIGHAGHPEVVGTMGQLPEGAVALIETVADAMAFQPRDPANVAFVTQTTLSVDDTAEIVEALRARFPAIAAPHKEDICYATTNRQEAVKAIAAKAQVLVVLGSANSSNSVRLAEVGRRAGARAYLIDDAEGLDFTWLEGVETVGVTAGASAPEVLVQGVLDRLAERFEVTLTEADTARETVTFKLPRALAG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000747
EMBL· GenBank· DDBJ
ACG79302.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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