B4J2W3 · WLS_DROGR

Function

function

A segment polarity gene required for wingless (wg)-dependent patterning processes, acting in both wg-sending cells and wg-target cells. In non-neuronal cells wls directs wg secretion. The wls traffic loop encompasses the Golgi, the cell surface, an endocytic compartment and a retrograde route leading back to the Golgi, and involves clathrin-mediated endocytosis and the retromer complex (a conserved protein complex consisting of Vps35 and Vps26). In neuronal cells (the larval motorneuron NMJ), the wg signal moves across the synapse via the release of wls-containing exosome-like vesicles. Postsynaptic wls is required for the trafficking of fz2 through the fz2-interacting protein Grip (By similarity).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcell projection
Cellular Componentearly endosome
Cellular Componentendoplasmic reticulum membrane
Cellular Componentendosome membrane
Cellular Componentextracellular exosome
Cellular ComponentGolgi membrane
Cellular Componentmultivesicular body
Cellular Componentneuromuscular junction
Cellular Componentplasma membrane
Cellular Componentpostsynaptic membrane
Cellular Componentpresynaptic membrane
Cellular Componentsynaptic vesicle membrane
Molecular FunctionWnt-protein binding
Biological Processcompound eye morphogenesis
Biological Processcuticle pattern formation
Biological ProcessGolgi to plasma membrane protein transport
Biological Processimaginal disc-derived leg morphogenesis
Biological Processimaginal disc-derived wing margin morphogenesis
Biological Processintracellular protein transport
Biological Processpositive regulation of protein secretion
Biological Processpositive regulation of Wnt protein secretion
Biological Processpositive regulation of Wnt signaling pathway
Biological Processregulation of intracellular protein transport
Biological Processsegment polarity determination
Biological Processtrans-synaptic signaling via exosome
Biological ProcessWnt protein secretion
Biological ProcessWnt signaling pathway

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Protein wntless

Gene names

    • Name
      wls
    • ORF names
      GH16661

Organism names

  • Taxonomic identifier
  • Strain
    • Tucson 15287-2541.00
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Hawaiian Drosophila

Accessions

  • Primary accession
    B4J2W3

Proteomes

Subcellular Location

Presynaptic cell membrane
; Multi-pass membrane protein
Postsynaptic cell membrane
; Multi-pass membrane protein
Cell membrane
; Multi-pass membrane protein
Endoplasmic reticulum membrane
; Multi-pass membrane protein
Endosome membrane
; Multi-pass membrane protein
Golgi apparatus membrane
; Multi-pass membrane protein
Note: In non-neuronal cells, wls binds to wg in the Golgi and accompanies it to the plasma membrane where the two proteins dissociate. Wg is secreted and wls is then internalized and returns to the Golgi apparatus in a retromer-dependent manner. Wls and wg colocalize in the Golgi apparatus in wg-producing cells, and reduced expression is seen in non-producing cells. Endoplasmic reticulum expression is unchanged in wg-producing versus non-producing cells. In neuronal cells, wls is localized both pre- and postsynaptically and is transferred trans-synaptically from the pre- to the postsynaptic compartment (By similarity).

Features

Showing features for topological domain, transmembrane.

Type
IDPosition(s)Description
Topological domain1-15Cytoplasmic
Transmembrane16-36Helical; Name=1
Topological domain37-239Lumenal
Transmembrane240-260Helical; Name=2
Topological domain261-275Cytoplasmic
Transmembrane276-296Helical; Name=3
Topological domain297-311Lumenal
Transmembrane312-332Helical; Name=4
Topological domain333-344Cytoplasmic
Transmembrane345-365Helical; Name=5
Topological domain366-390Lumenal
Transmembrane391-411Helical; Name=6
Topological domain412-441Cytoplasmic
Transmembrane442-462Helical; Name=7
Topological domain463-482Lumenal
Transmembrane483-503Helical; Name=8
Topological domain504-562Cytoplasmic

Keywords

PTM/Processing

Features

Showing features for chain, glycosylation.

Type
IDPosition(s)Description
ChainPRO_00003906631-562Protein wntless
Glycosylation58N-linked (GlcNAc...) asparagine
Glycosylation103N-linked (GlcNAc...) asparagine

Keywords

PTM databases

Interaction

Subunit

Interacts with wg; in the Golgi. Interacts with Vps35, a component of the retromer complex; wls stability is regulated by Vps35 (By similarity).

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the wntless family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    562
  • Mass (Da)
    64,187
  • Last updated
    2010-01-19 v2
  • Checksum
    885E4920290CB38C
MSGTILENLSGRKLSILVSSLMLCQVVCFLMGGLFAPVPAGHQTVLGSKCRDVPGRQNDTSFFLYSRGNGACKSLQDIDIEQDELKMANQLVYVFQMPLPRDNNTLQYSRWQQNLIGVLQVDIAYDSASELREPPKELQLTIDTRLAYRNQKDADTDWKLYAHSVEQRYLDCHASHVGRLETLYTCDIIPLFELGALHHNFYLLNLRFPMDTPKQMNLQFGHMHDLTLTAIHQNGGFTQVWLVLKTLLFPFVIGIMMWFWRRVHILQRSPALLEYMLFYLGGALSFLNLPLELLTLGVEMPYMLLLSDVRQGIFYAMLLSFWLVFAGEHMLIQDSPSKSTIRSRYWKHLSAVVVGCISLFVFDICERGVQMRNPFYSIWTTPLGAKVAMSFIVLAGVSAAIYFLFLCFMVWKVFKDIGDKRTSLPSMSQARRLHYEGLIYRFKFLMLATLLCAGLTVAGFIMGQMAEGHWKWNENIEIQLTSAFLTGVYGMWNIYIFALIILYAPSHKQWPTMRHSDETTQSNENIVASAASEEIEFSNLPSDSNPSEISSLTSFTRKVAFD

Sequence caution

The sequence EDV97133.1 differs from that shown. Reason: Erroneous gene model prediction

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CH916366
EMBL· GenBank· DDBJ
EDV97133.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.

Genome annotation databases

Similar Proteins

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