B4FK49 · NDK1_MAIZE
- ProteinNucleoside diphosphate kinase 1
- Genendpk1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids149 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (By similarity).
Involved in transcription regulation (Probable). Has G-quadruplex (G4) DNA-binding activity, which is independent of its nucleotide-binding and kinase activity. Binds folded G4 with low nanomolar affinity and corresponding unfolded G-rich DNA more weakly. Stabilizes folded G4s regardless of whether they are prefolded or not (PubMed:25679041).
Involved in transcription regulation (Probable). Has G-quadruplex (G4) DNA-binding activity, which is independent of its nucleotide-binding and kinase activity. Binds folded G4 with low nanomolar affinity and corresponding unfolded G-rich DNA more weakly. Stabilizes folded G4s regardless of whether they are prefolded or not (PubMed:25679041).
Catalytic activity
- a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP
Cofactor
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 9 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 57 | ATP (UniProtKB | ChEBI) | ||||
Sequence: F | ||||||
Binding site | 85 | ATP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 91 | ATP (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 102 | ATP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 112 | ATP (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Active site | 115 | Pros-phosphohistidine intermediate | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | DNA binding | |
Molecular Function | nucleoside diphosphate kinase activity | |
Biological Process | CTP biosynthetic process | |
Biological Process | GTP biosynthetic process | |
Biological Process | nucleoside triphosphate biosynthetic process | |
Biological Process | protein hexamerization | |
Biological Process | UTP biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNucleoside diphosphate kinase 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > PACMAD clade > Panicoideae > Andropogonodae > Andropogoneae > Tripsacinae > Zea
Accessions
- Primary accessionB4FK49
- Secondary accessions
Proteomes
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 115 | Loss of kinase activity. No effect on binding to prefolded G-quadruplex (G4) DNA or to unfolded G4 DNA. | ||||
Sequence: H → A | ||||||
Mutagenesis | 149 | No effect on kinase activity. 5-fold reduction in binding to prefolded G-quadruplex (G4) DNA. Loss fo binding to unfolded G4 DNA. | ||||
Sequence: K → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000445391 | 1-149 | Nucleoside diphosphate kinase 1 | |||
Sequence: MESTFIMIKPDGVQRGLIGEIISRFEKKGFYLKALKLVNVERSFAEKHYADLASKPFFQGLVDYIISGPVVAMVWEGKSVVTTGRKIIGATNPLASEPGTIRGDFAVDIGRNVIHGSDSIESANKEIALWFPEGPADWQSSQHPWIYEK |
Expression
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Length149
- Mass (Da)16,541
- Last updated2008-09-23 v1
- Checksum18980971F0474014
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A804LNF3 | A0A804LNF3_MAIZE | 100217209 | 155 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 135 | in Ref. 1; AIL33795 | ||||
Sequence: P → L |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KM347972 EMBL· GenBank· DDBJ | AIL33795.1 EMBL· GenBank· DDBJ | mRNA | ||
CM007647 EMBL· GenBank· DDBJ | ONL99892.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EU954075 EMBL· GenBank· DDBJ | ACG26193.1 EMBL· GenBank· DDBJ | mRNA | ||
EU954306 EMBL· GenBank· DDBJ | ACG26424.1 EMBL· GenBank· DDBJ | mRNA | ||
EU957798 EMBL· GenBank· DDBJ | ACG29916.1 EMBL· GenBank· DDBJ | mRNA | ||
EU961140 EMBL· GenBank· DDBJ | ACG33258.1 EMBL· GenBank· DDBJ | mRNA | ||
BT037487 EMBL· GenBank· DDBJ | ACF82492.1 EMBL· GenBank· DDBJ | mRNA | ||
BT061314 EMBL· GenBank· DDBJ | ACN26011.1 EMBL· GenBank· DDBJ | mRNA |