B4FK49 · NDK1_MAIZE

  • Protein
    Nucleoside diphosphate kinase 1
  • Gene
    ndpk1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (By similarity).
Involved in transcription regulation (Probable). Has G-quadruplex (G4) DNA-binding activity, which is independent of its nucleotide-binding and kinase activity. Binds folded G4 with low nanomolar affinity and corresponding unfolded G-rich DNA more weakly. Stabilizes folded G4s regardless of whether they are prefolded or not (PubMed:25679041).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site9ATP (UniProtKB | ChEBI)
Binding site57ATP (UniProtKB | ChEBI)
Binding site85ATP (UniProtKB | ChEBI)
Binding site91ATP (UniProtKB | ChEBI)
Binding site102ATP (UniProtKB | ChEBI)
Binding site112ATP (UniProtKB | ChEBI)
Active site115Pros-phosphohistidine intermediate

GO annotations

AspectTerm
Cellular Componentnucleus
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular Functionnucleoside diphosphate kinase activity
Biological ProcessCTP biosynthetic process
Biological ProcessGTP biosynthetic process
Biological Processnucleoside triphosphate biosynthetic process
Biological Processprotein hexamerization
Biological ProcessUTP biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Nucleoside diphosphate kinase 1
  • EC number
  • Alternative names
    • Nucleoside diphosphate kinase I
      (NDK I
      ; NDP kinase I
      ; NDPK I
      )
    • ZmNDPK1

Gene names

    • Name
      ndpk1
    • ORF names
      ZEAMMB73_Zm00001d029968

Organism names

  • Taxonomic identifier
  • Strains
    • W23
    • cv. B73
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > PACMAD clade > Panicoideae > Andropogonodae > Andropogoneae > Tripsacinae > Zea

Accessions

  • Primary accession
    B4FK49
  • Secondary accessions
    • A0A0A7DIX5

Proteomes

Genome annotation databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis115Loss of kinase activity. No effect on binding to prefolded G-quadruplex (G4) DNA or to unfolded G4 DNA.
Mutagenesis149No effect on kinase activity. 5-fold reduction in binding to prefolded G-quadruplex (G4) DNA. Loss fo binding to unfolded G4 DNA.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004453911-149Nucleoside diphosphate kinase 1

Expression

Gene expression databases

Interaction

Subunit

Homohexamer. Can also form dodecamers.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the NDK family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    149
  • Mass (Da)
    16,541
  • Last updated
    2008-09-23 v1
  • Checksum
    18980971F0474014
MESTFIMIKPDGVQRGLIGEIISRFEKKGFYLKALKLVNVERSFAEKHYADLASKPFFQGLVDYIISGPVVAMVWEGKSVVTTGRKIIGATNPLASEPGTIRGDFAVDIGRNVIHGSDSIESANKEIALWFPEGPADWQSSQHPWIYEK

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A804LNF3A0A804LNF3_MAIZE100217209155

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict135in Ref. 1; AIL33795

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KM347972
EMBL· GenBank· DDBJ
AIL33795.1
EMBL· GenBank· DDBJ
mRNA
CM007647
EMBL· GenBank· DDBJ
ONL99892.1
EMBL· GenBank· DDBJ
Genomic DNA
EU954075
EMBL· GenBank· DDBJ
ACG26193.1
EMBL· GenBank· DDBJ
mRNA
EU954306
EMBL· GenBank· DDBJ
ACG26424.1
EMBL· GenBank· DDBJ
mRNA
EU957798
EMBL· GenBank· DDBJ
ACG29916.1
EMBL· GenBank· DDBJ
mRNA
EU961140
EMBL· GenBank· DDBJ
ACG33258.1
EMBL· GenBank· DDBJ
mRNA
BT037487
EMBL· GenBank· DDBJ
ACF82492.1
EMBL· GenBank· DDBJ
mRNA
BT061314
EMBL· GenBank· DDBJ
ACN26011.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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