B4F769 · SMAL1_RAT

  • Protein
    SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
  • Gene
    Smarcal1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    5/5

Function

function

ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks (By similarity).

Features

Showing features for binding site.

1910100200300400500600700800900
TypeIDPosition(s)Description
Binding site415-422ATP (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular ComponentDNA replication factor A complex
Cellular Componentnuclear replication fork
Cellular Componentnucleoplasm
Cellular Componentnucleus
Cellular Componentsite of double-strand break
Molecular FunctionATP binding
Molecular FunctionATP-dependent activity, acting on DNA
Molecular FunctionATP-dependent DNA/DNA annealing activity
Molecular Functionhelicase activity
Molecular Functionhydrolase activity
Biological ProcessDNA damage response
Biological ProcessDNA repair
Biological Processdouble-strand break repair via nonhomologous end joining
Biological Processregulation of transcription by RNA polymerase II
Biological Processreplication fork processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
  • EC number
  • Alternative names
    • HepA-related protein
    • Sucrose nonfermenting protein 2-like 1

Gene names

    • Name
      Smarcal1
    • Synonyms
      Harp

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    B4F769

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain.

Type
IDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylserine
ChainPRO_00003615322-910SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
Modified residue117Phosphoserine
Modified residue164Phosphoserine

Post-translational modification

DNA damage-regulated phosphorylation by kinases that may include ATM, ATR and PRKDC.

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Interacts with RPA2; the interaction is direct and mediates the recruitment by the RPA complex of SMARCAL1 to sites of DNA damage.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, coiled coil, compositional bias, domain, motif.

Type
IDPosition(s)Description
Region1-170Disordered
Region2-30Mediates interaction with RPA2
Coiled coil3-28
Compositional bias7-29Basic and acidic residues
Compositional bias32-54Polar residues
Compositional bias70-95Polar residues
Compositional bias97-107Basic and acidic residues
Compositional bias120-131Polar residues
Compositional bias150-160Basic and acidic residues
Domain198-268HARP 1
Domain284-355HARP 2
Domain402-557Helicase ATP-binding
Motif506-509DESH box
Domain672-825Helicase C-terminal
Region865-890Disordered

Sequence similarities

Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    910
  • Mass (Da)
    101,212
  • Last updated
    2008-09-23 v1
  • MD5 Checksum
    38143DB4DF45C6C71CCC90BA8BBA34F8
MSLPLTEEQRKKIEENRQKALARRAEKLWAEQPQSTASGSSAARPSQCKQNSLLNLPAEPSKPEGHATISKGQNLNNSLPAAQRPHSSPCFQPSTAEEAKGLWKSEGKMSAACPNPSPPEVSNQQLLGSKSSEGHPQATQDTAASCPRPFPRDPKLEAKAGRPSTSGQSISDTFYALGEKTPKTDGRPAKALQTSPQKASCLRGMCLRTGDRFRVKIGYNKELIEVFKSLPSRRYDSFTKTWDFSMSDYRALMKAVERLSTVSLQPLEEVDGTGGQTSLPSAPSLTFVTGRCMLISRARFEVDIGYSEVVIALFKQMESRNYDPKTRKWNFLLEEHNKLIARSRELKQVQLDPLPKTLTLAFASQLEKTSLQSKADVPEADLSGVDAKLVSNLMPFQREGVSFAISKRGRLLLADDMGLGKTIQAICIAAFYRKEWPLLVVVPSSVRFTWEQAFLRWLPSLSPEDINVVVTGKGRLTAGLVNIVSFDLLSKLEKQLKTPFKVVIIDESHFLKNIKTARCRAAVPILKVAKRVILLSGTPAMSRPAELYTQIIAVKPTFFPQFHAFGLRYCDAKRLPWGWDYSGSSNLGELKLLLEEAVMLRRLKSDVLSQLPAKQRKMVVVNPGRISTRAKAALDAAAKEMTKDKTKQQQKEALLVFFNRTAEAKIPCVIEYILDLLESGREKFLVFAHHKVLLDAIAKELERKNVQHIRIDGSTPSADREDLCQQFQLSKGHTVAVLSITAANMGLTFSSADLVVFAELFWNPGVLIQAEDRVHRIGQTNSVGIHYLVAKGTADDYLWPLIQEKIKVLGEAGLSETNFSEMTEATDYLYKDPKQKTIYSLFQQSFEDDGNDMEFLEAAESFELGSTSGTSGNSSQELGDITDENALADSPPKKRRFEFFDNWDSFTSPF

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8L2QV60A0A8L2QV60_RATSmarcal1896

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias7-29Basic and acidic residues
Compositional bias32-54Polar residues
Compositional bias70-95Polar residues
Compositional bias97-107Basic and acidic residues
Compositional bias120-131Polar residues
Compositional bias150-160Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CH474004
EMBL· GenBank· DDBJ
EDL75309.1
EMBL· GenBank· DDBJ
Genomic DNA
BC168154
EMBL· GenBank· DDBJ
AAI68154.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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