B3VK56 · B3VK56_HUMAN
- ProteinNitric oxide synthase
- GeneNOS1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1434 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions.
Catalytic activity
- H+ + 2 L-arginine + 3 NADPH + 4 O2 = 4 H2O + 2 L-citrulline + 3 NADP+ + 2 nitric oxideThis reaction proceeds in the forward direction.
CHEBI:15378 + 2 CHEBI:32682 + 3 CHEBI:57783 + 4 CHEBI:15379 = 4 CHEBI:15377 + 2 CHEBI:57743 + 3 CHEBI:58349 + 2 CHEBI:16480
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 FAD.
Note: Binds 1 FMN.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | dendritic spine | |
Cellular Component | nucleoplasm | |
Cellular Component | plasma membrane | |
Cellular Component | sarcolemma | |
Molecular Function | calmodulin binding | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | FMN binding | |
Molecular Function | heme binding | |
Molecular Function | metal ion binding | |
Molecular Function | NADP binding | |
Molecular Function | nitric-oxide synthase activity | |
Biological Process | nitric oxide biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNitric oxide synthase
- EC number
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionB3VK56
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane, sarcolemma ; Peripheral membrane protein
PTM/Processing
Keywords
- PTM
Expression
Gene expression databases
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 17-99 | PDZ | ||||
Sequence: SVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGRPLVDLSYDSALEVLRGIASETHVVLILRG | ||||||
Region | 112-192 | Disordered | ||||
Sequence: TGDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAPRPPGQDPAKKAT | ||||||
Region | 276-302 | Disordered | ||||
Sequence: NNPYSEKEQPPTSGKQSPTKNGSPSKC | ||||||
Compositional bias | 280-299 | Polar residues | ||||
Sequence: SEKEQPPTSGKQSPTKNGSP | ||||||
Domain | 760-940 | Flavodoxin-like | ||||
Sequence: ATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVF | ||||||
Domain | 995-1242 | FAD-binding FR-type | ||||
Sequence: KRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLP |
Sequence similarities
Belongs to the NOS family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,434
- Mass (Da)160,970
- Last updated2008-09-02 v1
- Checksum99235793B953BF37
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 280-299 | Polar residues | ||||
Sequence: SEKEQPPTSGKQSPTKNGSP |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
EU753241 EMBL· GenBank· DDBJ | ACF04132.1 EMBL· GenBank· DDBJ | mRNA | ||
CH471054 EMBL· GenBank· DDBJ | EAW98110.1 EMBL· GenBank· DDBJ | Genomic DNA |