B3VA59 · AGLC_METVO
- ProteinDolichyl N-acetyl-alpha-D-glucosaminyl phosphate 3-beta-D-2,3-diacetamido-2,3-dideoxy-beta-D-glucuronosyltransferase
- GeneaglC
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids321 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in the assembly of an N-linked disaccharide that decorates the S-layer glycoprotein and flagellins (PubMed:18978056).
AglC catalyzes the transfer of 2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronic acid (Glc-2,3-diNAcA) from uridine 5'-diphospho 2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronic acid (UDP-Glc-2,3-diNAcA) to the AglK product Dol-P-GlcNAc to yield Dol-P-GlcNAc-Glc-2,3-diNAcA (PubMed:23624439).
AglC is specific for the monophosphate-linked Dol-P-GlcNAc (PubMed:23624439).
AglC catalyzes the transfer of 2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronic acid (Glc-2,3-diNAcA) from uridine 5'-diphospho 2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronic acid (UDP-Glc-2,3-diNAcA) to the AglK product Dol-P-GlcNAc to yield Dol-P-GlcNAc-Glc-2,3-diNAcA (PubMed:23624439).
AglC is specific for the monophosphate-linked Dol-P-GlcNAc (PubMed:23624439).
Catalytic activity
- an archaeal dolichyl N-acetyl-alpha-D-glucosaminyl phosphate + UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate = an archaeal dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy- beta-D-glucuronosyl)-N-acetyl- alpha-D-glucosaminyl phosphate + UDP + H+
an archaeal dolichyl N-acetyl-α-D-glucosaminyl phosphate RHEA-COMP:11931 + CHEBI:58745 = an archaeal dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy- β-D-glucuronosyl)-N-acetyl- α-D-glucosaminyl phosphate RHEA-COMP:12047 + CHEBI:58223 + CHEBI:15378
Pathway
Cell surface structure biogenesis; S-layer biogenesis.
Protein modification; protein glycosylation.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | hexosyltransferase activity | |
Biological Process | N-glycan processing | |
Biological Process | protein glycosylation | |
Biological Process | S-layer organization |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameDolichyl N-acetyl-alpha-D-glucosaminyl phosphate 3-beta-D-2,3-diacetamido-2,3-dideoxy-beta-D-glucuronosyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Methanomada group > Methanococci > Methanococcales > Methanococcaceae > Methanococcus
Accessions
- Primary accessionB3VA59
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 252-272 | Helical | |||
Transmembrane | 290-310 | Helical | |||
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Cells lacking this gene result in flagellins and S-layer proteins with significantly reduced apparent molecular masses, loss of flagellar assembly and absence of glycan attachment.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000435654 | 1-321 | Dolichyl N-acetyl-alpha-D-glucosaminyl phosphate 3-beta-D-2,3-diacetamido-2,3-dideoxy-beta-D-glucuronosyltransferase | ||
Structure
Sequence
- Sequence statusComplete
- Length321
- Mass (Da)37,328
- Last updated2008-09-02 v1
- Checksum4225C4DC1DB03D5A
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
EU726231 EMBL· GenBank· DDBJ | ACE74695.1 EMBL· GenBank· DDBJ | Genomic DNA |